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Literature summary extracted from

  • Lothier, J.; Van Laere, A.; Prudhomme, M.P.; Van den Ende, W.; Morvan-Bertrand, A.
    Cloning and characterization of a novel fructan 6-exohydrolase strongly inhibited by sucrose in Lolium perenne (2014), Planta, 240, 629-643.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.80 gene Lp6-FEHa, DNA and amino acid sequence determination and analysis, phylogenetic analysis, real-time quantitative PCR expression analysis, cloning in Escherichia coli, recombinant expression in Pichia pastoris strain X33, secretion of the protein to the culture medium Lolium perenne
3.2.1.154 cDNA library screening, gene Lp6-FEHa, DNA and amino acid sequence determination and analysis, phylogenetic analysis Lolium perenne

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.80 sucrose 70-90% inhibition at 10-40 mM Lolium perenne
3.2.1.154 sucrose the enzyme is 70-90% inhibited by 10-40 mM sucrose Lolium perenne

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.154 6,6-kestotetraose + H2O Lolium perenne
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.80 Lolium perenne D0PQE8 gene Lp6-FEHa
-
3.2.1.80 Lolium perenne Bravo D0PQE8 gene Lp6-FEHa
-
3.2.1.154 Lolium perenne D0PQE8
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.154 glycoprotein the enzyme contains five potential N-glycosylation sites Lolium perenne

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.80 recombinant extracellular enzyme from Pichia pastoris strain X33 from the culture supernatant Lolium perenne

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.80 leaf following defoliation, during fructan breakdown, the enzyme transcript level decreases in elongating leaf bases and in mature leaf sheaths in parallel to increased total fructan exohydrolase activities Lolium perenne
-
3.2.1.80 root
-
 Lolium perenne
-
3.2.1.80 stem stubble Lolium perenne
-
3.2.1.154 leaf in elongating leaf bases and mature leaf sheaths Lolium perenne
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.80 6,6-kestotetraose + H2O
-
 Lolium perenne ?
-
 ?
3.2.1.80 6,6-kestotetraose + H2O
-
 Lolium perenne Bravo ?
-
 ?
3.2.1.80 6-kestotriose + H2O best substrate Lolium perenne ?
-
 ?
3.2.1.80 6-kestotriose + H2O best substrate Lolium perenne Bravo ?
-
 ?
3.2.1.80 6G-kestotriose + H2O lower activity Lolium perenne ?
-
 ?
3.2.1.80 6G-kestotriose + H2O lower activity Lolium perenne Bravo ?
-
 ?
3.2.1.80 levan + H2O lower activity Lolium perenne ?
-
 ?
3.2.1.80 levan + H2O lower activity Lolium perenne Bravo ?
-
 ?
3.2.1.80 additional information substrate specificity, overview. Among beta(2,6) fructans tested, 6-kestotriose is the best substrate while bacterial levan and 6G-kestotriose are more slowly hydrolyzed. Among beta(2,1) fructans, inulin hydrolysis is neglectable while 1-kestotriose and 1,1-kestotetraose are also hydrolyzed but at a much lower rate than 6-kestotriose, poor activity with sucrose Lolium perenne ?
-
 ?
3.2.1.80 additional information substrate specificity, overview. Among beta(2,6) fructans tested, 6-kestotriose is the best substrate while bacterial levan and 6G-kestotriose are more slowly hydrolyzed. Among beta(2,1) fructans, inulin hydrolysis is neglectable while 1-kestotriose and 1,1-kestotetraose are also hydrolyzed but at a much lower rate than 6-kestotriose, poor activity with sucrose Lolium perenne Bravo ?
-
 ?
3.2.1.154 1,1-kestotetraose + H2O low activity Lolium perenne ?
-
 ?
3.2.1.154 1-kestotriose + H2O low activity Lolium perenne ?
-
 ?
3.2.1.154 6,6-kestotetraose + H2O
-
 Lolium perenne ?
-
 ?
3.2.1.154 6-kestotriose + H2O best substrate Lolium perenne ?
-
 ?
3.2.1.154 6G-kestotriose + H2O
-
 Lolium perenne ?
-
 ?
3.2.1.154 fructan + H2O
-
 Lolium perenne ?
-
 ?
3.2.1.154 levan + H2O from Erwinia herbicola Lolium perenne ?
-
 ?
3.2.1.154 additional information purification of and assay with fructans from Lolium perenne, overview Lolium perenne ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.80 6-FEH
-
 Lolium perenne
3.2.1.80 fructan 6-exohydrolase
-
 Lolium perenne
3.2.1.80 Lp6-FEHa
-
 Lolium perenne
3.2.1.154 6-FEH
-
 Lolium perenne
3.2.1.154 6-FEHa
-
 Lolium perenne
3.2.1.154 6-fructan exohydrolase
-
 Lolium perenne
3.2.1.154 Lp6-FEHa
-
 Lolium perenne

General Information

EC Number General Information Comment Organism
3.2.1.80 evolution the enzyme belongs to group I of glycosyl hydrolase famiyl 32, GH32, phylogenetic analysis, overview Lolium perenne
3.2.1.80 metabolism regulation during development of fructan exohydrolases, overview Lolium perenne
3.2.1.80 physiological function the enzyme is involved in the complex regulation of fructan breakdown in defoliated grassland species, which is a major forage grass species and accumulates fructans, mainly composed of beta(2,6)-linked fructose units. Fructans are mobilized through strongly increased activities of fructan exohydrolases Lolium perenne
3.2.1.154 evolution the enzyme's the catalytic triad is conserved among glycoside hydrolase family 32, GH32, members Lolium perenne
3.2.1.154 metabolism 6-FEHs are key enzymes associated with perennity of forage species Lolium perenne
3.2.1.154 physiological function Lolium perenne is a major forage grass species that accumulates fructans, mainly composed of beta(2,6)-linked fructose units. Fructans are mobilized through strongly increased activities of fructan exohydrolases (FEHs), sustaining regrowth following defoliation. Differences in the regulation of FEH activity among forage grasses influence their tolerance to defoliation Lolium perenne