Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hanano, A.; Harba, M.; Al-Ali, M.; Ammouneh, H.
    Silencing of Erwinia amylovora sy69 AHL-quorum sensing by a Bacillus simplex AHL-inducible aiiA gene encoding a zinc-dependent N-acyl-homoserine lactonase (2014), Plant Pathol., 63, 773-783.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.81 expressed in Escherichia coli as a His-tagged fusion protein Peribacillus simplex

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.81 Zn2+ required Peribacillus simplex

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.81 28000
-
 SDS-PAGE Peribacillus simplex

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.81 Peribacillus simplex
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.81 using Ni-NTA chromatography Peribacillus simplex

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.81 N-(3-oxo-N-hexanoyl)-L-homoserine lactone + H2O relative activity: 97.2% Peribacillus simplex N-(3-oxo-N-hexanoyl)-L-homoserine
-
 ?
3.1.1.81 N-(3-oxo-octanoyl)-L-homoserine lactone + H2O relative activity: 100% Peribacillus simplex N-(3-oxo-octanoyl)-L-homoserine
-
 ?
3.1.1.81 N-butanoyl-L-homoserine lactone + H2O weak substrate Peribacillus simplex N-butanoyl-L-homoserine
-
 ?
3.1.1.81 N-hexanoyl-L-homoserine lactone + H2O
-
 Peribacillus simplex N-hexanoyl-L-homoserine
-
 ?
3.1.1.81 N-octanoyl-L-homoserine lactone + H2O relative activity: 98.9% Peribacillus simplex N-octanoyl-L-homoserine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.81 AHL-lactonase
-
 Peribacillus simplex
3.1.1.81 AiiA
-
 Peribacillus simplex

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.81 28
-
 assay at Peribacillus simplex

Expression

EC Number Organism Comment Expression
3.1.1.81 Peribacillus simplex expression of the aiiA gene is significantly up-regulated by 3-oxo-AHL up

General Information

EC Number General Information Comment Organism
3.1.1.81 physiological function AHL-lactonase inhibits multiplication of the N-(3-oxo-N-hexanoyl) L-homoserine lactone-producing plant pathogen Erwinia amylovora sy69 both in vitro and in planta Peribacillus simplex