EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.182 | phylogenetic analysis, heterologus expression | Consolida orientalis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.182 | 4.6 | - |
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside | recombinant enzyme, pH and temperature not specified in the publication | Consolida orientalis | |
3.2.1.182 | 4.8 | - |
Dhurrin | recombinant enzyme, pH and temperature not specified in the publication | Consolida orientalis | |
3.2.1.182 | 28.4 | - |
(2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside | recombinant enzyme, pH and temperature not specified in the publication | Consolida orientalis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.182 | (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O | Consolida orientalis | - |
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-ol + D-glucose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.182 | Consolida orientalis | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.182 | flower | - |
Consolida orientalis | - |
3.2.1.182 | flower bud | - |
Consolida orientalis | - |
3.2.1.182 | leaf | of all ages | Consolida orientalis | - |
3.2.1.182 | additional information | no expression in roots | Consolida orientalis | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.182 | (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O | - |
Consolida orientalis | (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-ol + D-glucose | - |
? | |
3.2.1.182 | (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O | preferred substrate | Consolida orientalis | (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-ol + D-glucose | - |
? | |
3.2.1.182 | (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O | low activity | Consolida orientalis | (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-ol + D-glucose | - |
? | |
3.2.1.182 | dhurrin + H2O | - |
Consolida orientalis | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.182 | Co-Glu | - |
Consolida orientalis |
3.2.1.182 | GDIBOA-glucosidase | - |
Consolida orientalis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.182 | 4.2 | - |
(2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside | recombinant enzyme, pH and temperature not specified in the publication | Consolida orientalis | |
3.2.1.182 | 32 | - |
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside | recombinant enzyme, pH and temperature not specified in the publication | Consolida orientalis | |
3.2.1.182 | 52 | - |
Dhurrin | recombinant enzyme, pH and temperature not specified in the publication | Consolida orientalis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.2.1.182 | evolution | the enzyme belongs to the glycoside hydrolase family 1, GH1, phylogenetic analysis, overview | Consolida orientalis |
3.2.1.182 | metabolism | the enzyme is involved in the benzoxazinoid pathway, overview | Consolida orientalis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.182 | 0.15 | - |
(2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside | recombinant enzyme, pH and temperature not specified in the publication | Consolida orientalis | |
3.2.1.182 | 7 | - |
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside | recombinant enzyme, pH and temperature not specified in the publication | Consolida orientalis | |
3.2.1.182 | 10.8 | - |
Dhurrin | recombinant enzyme, pH and temperature not specified in the publication | Consolida orientalis |