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Literature summary extracted from

  • Dick, R.; Rattei, T.; Haslbeck, M.; Schwab, W.; Gierl, A.; Frey, M.
    Comparative analysis of benzoxazinoid biosynthesis in monocots and dicots: independent recruitment of stabilization and activation functions (2012), Plant Cell, 24, 915-928.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.182 phylogenetic analysis, heterologus expression Consolida orientalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.182 4.6
-
 (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside recombinant enzyme, pH and temperature not specified in the publication Consolida orientalis
3.2.1.182 4.8
-
 Dhurrin recombinant enzyme, pH and temperature not specified in the publication Consolida orientalis
3.2.1.182 28.4
-
 (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside recombinant enzyme, pH and temperature not specified in the publication Consolida orientalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.182 (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O Consolida orientalis
-
 (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-ol + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.182 Consolida orientalis
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.182 flower
-
 Consolida orientalis
-
3.2.1.182 flower bud
-
 Consolida orientalis
-
3.2.1.182 leaf of all ages Consolida orientalis
-
3.2.1.182 additional information no expression in roots Consolida orientalis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.182 (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O
-
 Consolida orientalis (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-ol + D-glucose
-
 ?
3.2.1.182 (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O preferred substrate Consolida orientalis (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-ol + D-glucose
-
 ?
3.2.1.182 (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O low activity Consolida orientalis (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-ol + D-glucose
-
 ?
3.2.1.182 dhurrin + H2O
-
 Consolida orientalis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.182 Co-Glu
-
 Consolida orientalis
3.2.1.182 GDIBOA-glucosidase
-
 Consolida orientalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.182 4.2
-
 (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside recombinant enzyme, pH and temperature not specified in the publication Consolida orientalis
3.2.1.182 32
-
 (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside recombinant enzyme, pH and temperature not specified in the publication Consolida orientalis
3.2.1.182 52
-
 Dhurrin recombinant enzyme, pH and temperature not specified in the publication Consolida orientalis

General Information

EC Number General Information Comment Organism
3.2.1.182 evolution the enzyme belongs to the glycoside hydrolase family 1, GH1, phylogenetic analysis, overview Consolida orientalis
3.2.1.182 metabolism the enzyme is involved in the benzoxazinoid pathway, overview Consolida orientalis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.182 0.15
-
 (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside recombinant enzyme, pH and temperature not specified in the publication Consolida orientalis
3.2.1.182 7
-
 (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside recombinant enzyme, pH and temperature not specified in the publication Consolida orientalis
3.2.1.182 10.8
-
 Dhurrin recombinant enzyme, pH and temperature not specified in the publication Consolida orientalis