BRENDA - Enzyme Database

Comparative analysis of benzoxazinoid biosynthesis in monocots and dicots: independent recruitment of stabilization and activation functions

Dick, R.; Rattei, T.; Haslbeck, M.; Schwab, W.; Gierl, A.; Frey, M.; Plant Cell 24, 915-928 (2012)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
3.2.1.182
phylogenetic analysis, heterologus expression
Consolida orientalis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.182
4.6
-
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside
recombinant enzyme, pH and temperature not specified in the publication
Consolida orientalis
3.2.1.182
4.8
-
Dhurrin
recombinant enzyme, pH and temperature not specified in the publication
Consolida orientalis
3.2.1.182
28.4
-
(2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside
recombinant enzyme, pH and temperature not specified in the publication
Consolida orientalis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.2.1.182
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O
Consolida orientalis
-
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-ol + D-glucose
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.2.1.182
Consolida orientalis
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.182
flower
-
Consolida orientalis
-
3.2.1.182
flower bud
-
Consolida orientalis
-
3.2.1.182
leaf
of all ages
Consolida orientalis
-
3.2.1.182
additional information
no expression in roots
Consolida orientalis
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.2.1.182
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O
-
732629
Consolida orientalis
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-ol + D-glucose
-
-
-
?
3.2.1.182
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O
preferred substrate
732629
Consolida orientalis
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-ol + D-glucose
-
-
-
?
3.2.1.182
(2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O
low activity
732629
Consolida orientalis
(2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-ol + D-glucose
-
-
-
?
3.2.1.182
dhurrin + H2O
-
732629
Consolida orientalis
?
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
3.2.1.182
Co-Glu
-
Consolida orientalis
3.2.1.182
GDIBOA-glucosidase
-
Consolida orientalis
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2.1.182
4.2
-
(2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside
recombinant enzyme, pH and temperature not specified in the publication
Consolida orientalis
3.2.1.182
32
-
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside
recombinant enzyme, pH and temperature not specified in the publication
Consolida orientalis
3.2.1.182
52
-
Dhurrin
recombinant enzyme, pH and temperature not specified in the publication
Consolida orientalis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.182
phylogenetic analysis, heterologus expression
Consolida orientalis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.182
4.6
-
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside
recombinant enzyme, pH and temperature not specified in the publication
Consolida orientalis
3.2.1.182
4.8
-
Dhurrin
recombinant enzyme, pH and temperature not specified in the publication
Consolida orientalis
3.2.1.182
28.4
-
(2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside
recombinant enzyme, pH and temperature not specified in the publication
Consolida orientalis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.2.1.182
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O
Consolida orientalis
-
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-ol + D-glucose
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.182
flower
-
Consolida orientalis
-
3.2.1.182
flower bud
-
Consolida orientalis
-
3.2.1.182
leaf
of all ages
Consolida orientalis
-
3.2.1.182
additional information
no expression in roots
Consolida orientalis
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.2.1.182
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O
-
732629
Consolida orientalis
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-ol + D-glucose
-
-
-
?
3.2.1.182
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O
preferred substrate
732629
Consolida orientalis
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-ol + D-glucose
-
-
-
?
3.2.1.182
(2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O
low activity
732629
Consolida orientalis
(2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-ol + D-glucose
-
-
-
?
3.2.1.182
dhurrin + H2O
-
732629
Consolida orientalis
?
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2.1.182
4.2
-
(2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside
recombinant enzyme, pH and temperature not specified in the publication
Consolida orientalis
3.2.1.182
32
-
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside
recombinant enzyme, pH and temperature not specified in the publication
Consolida orientalis
3.2.1.182
52
-
Dhurrin
recombinant enzyme, pH and temperature not specified in the publication
Consolida orientalis
General Information
EC Number
General Information
Commentary
Organism
3.2.1.182
evolution
the enzyme belongs to the glycoside hydrolase family 1, GH1, phylogenetic analysis, overview
Consolida orientalis
3.2.1.182
metabolism
the enzyme is involved in the benzoxazinoid pathway, overview
Consolida orientalis
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.2.1.182
evolution
the enzyme belongs to the glycoside hydrolase family 1, GH1, phylogenetic analysis, overview
Consolida orientalis
3.2.1.182
metabolism
the enzyme is involved in the benzoxazinoid pathway, overview
Consolida orientalis
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.2.1.182
0.15
-
(2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside
recombinant enzyme, pH and temperature not specified in the publication
Consolida orientalis
3.2.1.182
7
-
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside
recombinant enzyme, pH and temperature not specified in the publication
Consolida orientalis
3.2.1.182
10.8
-
Dhurrin
recombinant enzyme, pH and temperature not specified in the publication
Consolida orientalis
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.2.1.182
0.15
-
(2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside
recombinant enzyme, pH and temperature not specified in the publication
Consolida orientalis
3.2.1.182
7
-
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside
recombinant enzyme, pH and temperature not specified in the publication
Consolida orientalis
3.2.1.182
10.8
-
Dhurrin
recombinant enzyme, pH and temperature not specified in the publication
Consolida orientalis