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Literature summary extracted from

  • Carelli, M.; Biazzi, E.; Panara, F.; Tava, A.; Scaramelli, L.; Porceddu, A.; Graham, N.; Odoardi, M.; Piano, E.; Arcioni, S.; May, S.; Scotti, C.; Calderini, O.
    Medicago truncatula CYP716A12 is a multifunctional oxidase involved in the biosynthesis of hemolytic saponins (2011), Plant Cell, 23, 3070-3081.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.126 expression in Saccharomyces cerevisiae Medicago truncatula

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.126 microsome
-
 Medicago truncatula
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.126 57000
-
 x * 57000, SDS-PAGE Medicago truncatula

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.126 Medicago truncatula F8J4R7
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.126 flower highest expression of the gene Medicago truncatula
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1.14.14.126 leaf significant increase in expression is observed in the reproductive stages, with a maximum expression at flowering Medicago truncatula
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1.14.14.126 additional information expression of the gene occurs in all the organs and the developmental stages Medicago truncatula
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1.14.14.126 root
-
 Medicago truncatula
-
1.14.14.126 stem
-
 Medicago truncatula
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.126 beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 overall reaction Medicago truncatula oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O CYP716A12 mainly catalyzes the sequential three-step oxidation at the C-28 position necessary to transform beta-amyrin into oleanolic acid ?
1.14.14.126 erythrodiol + [reduced NADPH-hemoprotein reductase] + O2
-
 Medicago truncatula oleanolic aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.126 ? x * 57000, SDS-PAGE Medicago truncatula

Synonyms

EC Number Synonyms Comment Organism
1.14.14.126 CYP716A12
-
 Medicago truncatula

General Information

EC Number General Information Comment Organism
1.14.14.126 physiological function enzyme is responsible for an early step in the saponin biosynthetic pathway. Mutants in CYP716A12 are unable to produce hemolytic saponins and only synthetize soyasaponins. CYP716A12 catalyzes the oxidation of beta-amyrin and erythrodiol at the C-28 position, yielding oleanolic acid Medicago truncatula