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Literature summary extracted from

  • Loebers, A.; Mueller-Uri, F.; Kreis, W.
    A young root-specific gene (ArMY2) from horseradish encoding a MYR II myrosinase with kinetic preference for the root-specific glucosinolate gluconasturtiin (2014), Phytochemistry, 99, 26-35.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.147 phylogenetic tree, recombinant overexpression of His6-tagged ArMy2 in Pichia pastoris Armoracia rusticana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.147 0.11
-
 gluconasturtiin recombinant enzyme, pH 5.5, 37°C Armoracia rusticana
3.2.1.147 0.53
-
 sinigrin recombinant enzyme, pH 5.5, 37°C Armoracia rusticana
3.2.1.147 16.81
-
 4-nitrophenyl beta-D-glucopyranoside recombinant enzyme, pH 5.5, 37°C Armoracia rusticana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.147 57000
-
 x * 57000, SDS-PAGE Armoracia rusticana

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.147 Armoracia rusticana I1TIJ2 young root-specific gene ArMY2
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.147 glycoprotein the enzyme ArMy2 possesses 5 putative N-glycosylation sites, degree of glycosylation is about 19% Armoracia rusticana

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.147 recombinant His6-tagged ArMy2 from Pichia pastoris by nickel affinity chromatography Armoracia rusticana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.147 leaf
-
 Armoracia rusticana
-
3.2.1.147 additional information glucosinolate contents in young and storage root and in leaves, overview Armoracia rusticana
-
3.2.1.147 root
-
 Armoracia rusticana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.147 4-nitrophenyl beta-D-glucopyranoside + H2O a poor substrate for ArMy2 Armoracia rusticana 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.147 glucobrassicin + H2O
-
 Armoracia rusticana ?
-
 ?
3.2.1.147 gluconasturtiin + H2O
-
 Armoracia rusticana ?
-
 ?
3.2.1.147 additional information myrosinase-catalyzed deglycosylation of a glucosinolate releases beta-D-glucose and an unstable intermediate, which undergoes rearrangements to finally yield an isothiocyanate Armoracia rusticana ?
-
 ?
3.2.1.147 sinigrin + H2O
-
 Armoracia rusticana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.147 ? x * 57000, SDS-PAGE Armoracia rusticana

Synonyms

EC Number Synonyms Comment Organism
3.2.1.147 ArMy2
-
 Armoracia rusticana
3.2.1.147 MYR II
-
 Armoracia rusticana
3.2.1.147 myrosinase
-
 Armoracia rusticana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.147 28
-
-
 Armoracia rusticana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.147 4.21
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.5, 37°C Armoracia rusticana
3.2.1.147 8.91
-
 gluconasturtiin pH 5.5, 37°C Armoracia rusticana
3.2.1.147 10.69
-
 sinigrin pH 5.5, 37°C Armoracia rusticana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.147 5 5.5 rather sharp maximum between pH 5.0-5.5 Armoracia rusticana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.147 0.26
-
 4-nitrophenyl beta-D-glucopyranoside recombinant enzyme, pH 5.5, 37°C Armoracia rusticana
3.2.1.147 20.16
-
 sinigrin recombinant enzyme, pH 5.5, 37°C Armoracia rusticana
3.2.1.147 83.13
-
 gluconasturtiin recombinant enzyme, pH 5.5, 37°C Armoracia rusticana