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Literature summary extracted from

  • Meyer, J.G.; Kim, S.; Maltby, D.A.; Ghassemian, M.; Bandeira, N.; Komives, E.A.
    Expanding proteome coverage with orthogonal-specificity alpha-lytic proteases (2014), Mol. Cell. Proteomics, 13, 823-835.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.12 sodium deoxycholate 196% activity in the presence of 0.1% (w/v) sodium deoxycholate Lysobacter enzymogenes

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.12 M190A active site mutant Lysobacter enzymogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.12 guanidine hydrochloride 1% residual activity in the presence of 4 mM guanidine hydrochloride Lysobacter enzymogenes
3.4.21.12 SDS 42% residual activity in the presence of 1% (w/v) SDS Lysobacter enzymogenes
3.4.21.12 Urea 12% residual activity in the presence of 4 mM urea Lysobacter enzymogenes

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.12 Lysobacter enzymogenes
-
-
-
3.4.21.12 Lysobacter enzymogenes 495
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.12 N-succinyl-L-Ala-L-Ala-L-Ala 4-nitroanilide + H2O
-
 Lysobacter enzymogenes N-succinyl-L-Ala-L-Ala-L-Ala + 4-nitroaniline
-
 ?
3.4.21.12 N-succinyl-L-Ala-L-Ala-L-Ala 4-nitroanilide + H2O
-
 Lysobacter enzymogenes 495 N-succinyl-L-Ala-L-Ala-L-Ala + 4-nitroaniline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.12 alpha-lytic protease
-
 Lysobacter enzymogenes