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Literature summary extracted from

  • Zhuravleva, E.; Gut, H.; Hynx, D.; Marcellin, D.; Bleck, C.K.; Genoud, C.; Cron, P.; Keusch, J.J.; Dummler, B.; Esposti, M.D.; Hemmings, B.A.
    Acyl coenzyme A thioesterase Them5/Acot15 is involved in cardiolipin remodeling and fatty liver development (2012), Mol. Cell. Biol., 32, 2685-2697.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.20 analysis carried out with a deletion mutant lacking the first 35 amino acids DELTA1-34. Expressed in Escherichia coli as a His-tagged fusion protein Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.2.20 enzyme forms homodimers in crystals. DELTA1-34 deletion mutant is crystallized to 1.45 A resolution Homo sapiens
3.1.2.20 enzyme forms homodimers in crystals. DELTA1-34 deletion mutant is crystallized to 1.6 A resolution Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.2.20 D167A D167 is a key catalytic residue. D167A no longer displays catalytic activity Homo sapiens
3.1.2.20 DELTA1-37 mutant lacking the first 37 N-terminal amino acids is excluded from mitochondria Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.20 0.0024
-
 palmitoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
3.1.2.20 0.0029
-
 vaccenoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
3.1.2.20 0.0042
-
 stearoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
3.1.2.20 0.0042
-
 linoleoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
3.1.2.20 0.0086
-
 myristoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
3.1.2.20 0.0154
-
 linolenoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
3.1.2.20 0.019
-
 Arachidonoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
3.1.2.20 0.026
-
 palmitoleoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.20 mitochondrion
-
 Mus musculus 5739
-
3.1.2.20 mitochondrion
-
 Homo sapiens 5739
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.20 Homo sapiens Q5T1C6
-
-
3.1.2.20 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.20 using Ni-NTA chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.20 arachidonoyl-CoA + H2O
-
 Homo sapiens CoA + arachidonate
-
 ?
3.1.2.20 linolenoyl-CoA + H2O
-
 Homo sapiens CoA + linolenoate
-
 ?
3.1.2.20 linoleoyl-CoA + H2O
-
 Homo sapiens CoA + linolate
-
 ?
3.1.2.20 myristoyl-CoA + H2O
-
 Homo sapiens CoA + myristic acid
-
 ?
3.1.2.20 palmitoleoyl-CoA + H2O
-
 Homo sapiens CoA + palmitoleoate
-
 ?
3.1.2.20 palmitoyl-CoA + H2O
-
 Homo sapiens CoA + palmitate
-
 ?
3.1.2.20 stearoyl-CoA + H2O
-
 Homo sapiens CoA + stearate
-
 ?
3.1.2.20 vaccenoyl-CoA + H2O
-
 Homo sapiens CoA + vaccenoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.20 dimer gel filtration Homo sapiens
3.1.2.20 homodimer gel filtration Homo sapiens
3.1.2.20 homotetramer gel filtration, only minor fraction Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.1.2.20 acyl-CoA thioesterase
-
 Mus musculus
3.1.2.20 acyl-CoA thioesterase
-
 Homo sapiens
3.1.2.20 Them4
-
 Homo sapiens
3.1.2.20 Them5
-
 Mus musculus
3.1.2.20 Them5
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.20 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.2.20 0.2
-
 stearoyl-CoA pH 7.5, 37°C, mutant D167A Homo sapiens
3.1.2.20 0.5
-
 palmitoyl-CoA pH 7.5, 37°C, mutant D167A Homo sapiens
3.1.2.20 0.8
-
 linoleoyl-CoA pH 7.5, 37°C, mutant D167A Homo sapiens
3.1.2.20 1.2
-
 stearoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
3.1.2.20 1.4
-
 Arachidonoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
3.1.2.20 1.8
-
 vaccenoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
3.1.2.20 11.5
-
 palmitoleoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
3.1.2.20 12.2
-
 linoleoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
3.1.2.20 13.3
-
 linolenoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
3.1.2.20 16.6
-
 palmitoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
3.1.2.20 19.7
-
 myristoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens

General Information

EC Number General Information Comment Organism
3.1.2.20 malfunction Them5 knockout mice display changes in the lipid profile of cardiolipin and its metabolites. Them5 loss results in an 2fold decrease in free fatty acids in mitochondria, with a particularly marked decrease in linoleic and linolenic acids in the knockout samples. Them5 knockout mice develop fatty liver. In addition, loss of thioesterase activity of Them5 leads to changes in mitochondrial morphology and function Mus musculus
3.1.2.20 malfunction Them5-/- mice show deregulation of lipid metabolism and the development of fatty liver, exacerbated by a high-fat diet. Mitochondrial morphology is affected, and functions such as respiration and beta-oxidation are impaired Mus musculus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.2.20 73
-
 Arachidonoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
3.1.2.20 290
-
 stearoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
3.1.2.20 430
-
 palmitoleoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
3.1.2.20 540
-
 vaccenoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
3.1.2.20 860
-
 linolenoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
3.1.2.20 2300
-
 myristoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
3.1.2.20 2900
-
 linoleoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
3.1.2.20 6900
-
 palmitoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens