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Literature summary extracted from

  • Ghosh, A.; Verma, A.K.; Tingirikari, J.R.; Shukla, R.; Goyal, A.
    Recovery and purification of oligosaccharides from copra meal by recombinant endo-beta-mannanase and deciphering molecular mechanism involved and its role as potent therapeutic agent (2015), Mol. Biotechnol., 57, 111-127.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.78 medicine mixed maltooligosacchrides produced by the enzyme exhibit in vitro anti-tumorigenic activity on human colon cancer HT29 cells Acetivibrio thermocellus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Acetivibrio thermocellus
-
-
-
3.2.1.78 Acetivibrio thermocellus ATCC 27405
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 additional information production of mannooligosaccharides from pretreated and defatted copra meal hydrolysis by the enzyme Acetivibrio thermocellus ?
-
 ?
3.2.1.78 additional information production of mannooligosaccharides from pretreated and defatted copra meal hydrolysis by the enzyme Acetivibrio thermocellus ATCC 27405 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.78 More the three-dimensional enzyme structure contains a (beta/alpha)8 TIM barrel folding motif Acetivibrio thermocellus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.78 CtManf
-
 Acetivibrio thermocellus
3.2.1.78 endo-beta-(1->4)-mannanase
-
 Acetivibrio thermocellus
3.2.1.78 endo-beta-mannanase
-
 Acetivibrio thermocellus
3.2.1.78 endo-mannanase
-
 Acetivibrio thermocellus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 60
-
 assay at Acetivibrio thermocellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 6.9
-
 assay at Acetivibrio thermocellus

General Information

EC Number General Information Comment Organism
3.2.1.78 additional information catalytic residues are Glu181 as catalytic acid/base and Glu288 as nucleophile, molecular docking study with different manno-configured ligands from mannobiose to mannohexose as well as galactomannan. The ability to accommodate larger ligand molecules in the active site of CtManT is probably due to the long loops enclosing the active site that provides the depth of the cavity, structure overview Acetivibrio thermocellus