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Literature summary extracted from

  • Zawistowska-Deniziak, A.; Wasyl, K.; Norbury, L.J.; Wesolowska, A.; Bien, J.; Grodzik, M.; Wisniewski, M.; Baska, P.; Wedrychowicz, H.
    Characterization and differential expression of cathepsin L3 alleles from Fasciola hepatica (2013), Mol. Biochem. Parasitol., 190, 27-37.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.B62 expressed in Escherichia coli BL21 and Pichia pastoris X33 cells Fasciola hepatica

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.B62 E-64
-
 Fasciola hepatica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.B62 38080
-
 x * 38080, isoform CL3-1, calculated from amino acid sequence Fasciola hepatica
3.4.22.B62 38280
-
 x * 38280, isoform CL3-2, calculated from amino acid sequence Fasciola hepatica
3.4.22.B62 46000
-
 x * 46000, recombinant proenzyme, SDS-PAGE Fasciola hepatica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.B62 Collagen + H2O Fasciola hepatica
-
 ?
-
 ?
3.4.22.B62 human immumglobulin G + H2O Fasciola hepatica partial cleavage ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.B62 Fasciola hepatica B9VXS1 isoform CL3-2
-
3.4.22.B62 Fasciola hepatica B9VXS2 isoform CL3-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.B62 nickel affinity column chromatography Fasciola hepatica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.B62 juvenile newly excysted Fasciola hepatica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.B62 Collagen + H2O
-
 Fasciola hepatica ?
-
 ?
3.4.22.B62 human immumglobulin G + H2O partial cleavage Fasciola hepatica ?
-
 ?
3.4.22.B62 tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
 Fasciola hepatica tosyl-Gly-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.B62 ? x * 38080, isoform CL3-1, calculated from amino acid sequence Fasciola hepatica
3.4.22.B62 ? x * 38280, isoform CL3-2, calculated from amino acid sequence Fasciola hepatica
3.4.22.B62 ? x * 46000, recombinant proenzyme, SDS-PAGE Fasciola hepatica

Synonyms

EC Number Synonyms Comment Organism
3.4.22.B62 CL3
-
 Fasciola hepatica
3.4.22.B62 CL3-1 isoform with high expression Fasciola hepatica
3.4.22.B62 CL3-2 isoform with low expression Fasciola hepatica

Expression

EC Number Organism Comment Expression
3.4.22.B62 Fasciola hepatica maximum expression level is observed in newly excysted juveniles immediately after excystment up