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Literature summary extracted from

  • Vinoj, G.; Vaseeharan, B.; Thomas, S.; Spiers, A.; Shanthi, S.
    Quorum-quenching activity of the AHL-lactonase from Bacillus licheniformis DAHB1 inhibits Vibrio biofilm formation in vitro and reduces shrimp intestinal colonisation and mortality (2014), Mar. Biotechnol., 16, 707-715.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.81 expressed in Escherichia coli as a His-tagged fusion protein Bacillus licheniformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.81 28000
-
 calculated from cDNA Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.81 Bacillus licheniformis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.81 using Ni-NTA chromatography Bacillus licheniformis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.81 N-(3-oxo-N-hexanoyl)-L-homoserine lactone + H2O
-
 Bacillus licheniformis N-(3-oxo-N-hexanoyl)-L-homoserine
-
 ?
3.1.1.81 N-(3-oxo-octanoyl)-L-homoserine lactone + H2O
-
 Bacillus licheniformis N-(3-oxo-octanoyl)-L-homoserine
-
 ?
3.1.1.81 N-butanoyl-L-homoserine lactone + H2O
-
 Bacillus licheniformis N-butanoyl-L-homoserine
-
 ?
3.1.1.81 N-decanoyl-L-homoserine lactone + H2O
-
 Bacillus licheniformis N-decanoyl-L-homoserine
-
 ?
3.1.1.81 N-dodecanoyl-L-homoserine lactone + H2O
-
 Bacillus licheniformis N-dodecanoyl-L-homoserine
-
 ?
3.1.1.81 N-hexanoyl-L-homoserine lactone + H2O
-
 Bacillus licheniformis N-hexanoyl-L-homoserine
-
 ?
3.1.1.81 N-octanoyl-L-homoserine lactone + H2O
-
 Bacillus licheniformis N-octanoyl-L-homoserine
-
 ?
3.1.1.81 N-tetradecanoyl-L-homoserine lactone + H2O
-
 Bacillus licheniformis N-tetradecanoyl-L-homoserine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.81 AHL-lactonase
-
 Bacillus licheniformis
3.1.1.81 AiiA
-
 Bacillus licheniformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.81 25
-
 assay at Bacillus licheniformis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.81 30 50
-
 Bacillus licheniformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.81 60 80 retains 90% activity after incubation at 60-80 °C for 60min Bacillus licheniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.81 8
-
 assay at Bacillus licheniformis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.81 7 8
-
 Bacillus licheniformis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.81 6 12 retains more than 80% of the initial activity after incubation at pH 6.0-12.0 for 60 min Bacillus licheniformis

General Information

EC Number General Information Comment Organism
3.1.1.81 physiological function purified recombinant AiiA inhibits vibrio biofilm development in a cover slip assay and significantly attenuates infection and mortality in shrimps reared in a recirculation aquaculture system Bacillus licheniformis