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Literature summary extracted from

  • Abraham, A.; Narayanan, S.; Philip, S.; Nair, D.; Chandrasekharan, A.; Kochupurackal, J.
    In silico characterization of a novel beta-1,3-glucanase gene from Bacillus amyloliquefaciens - A bacterial endophyte of Hevea brasiliensis antagonistic to Phytophthora meadii (2013), J. Mol. Model., 19, 999-1007.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.39 DNA and amino acid sequence determination and analysis, phylogenetic tree and sequence comparisons Bacillus amyloliquefaciens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.39 structure homology modelling Bacillus amyloliquefaciens

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.39 I157L site-directed mutagenesis of the active site residue Bacillus amyloliquefaciens
3.2.1.39 additional information mutant docking study, overview Bacillus amyloliquefaciens
3.2.1.39 N52D site-directed mutagenesis of the active site residue Bacillus amyloliquefaciens
3.2.1.39 V158A site-directed mutagenesis of the active site residue Bacillus amyloliquefaciens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.39 extracellular the enzyme is secreted, it contains a cleavage site exists between amino acid positions 25 and 26 Bacillus amyloliquefaciens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.39 27790
-
 x * 27790, sequence calculation Bacillus amyloliquefaciens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Bacillus amyloliquefaciens
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 endophytic in Hevea brasiliensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.39 53 76 different isolates, pH and temperature not specified in the publication Bacillus amyloliquefaciens

Subunits

EC Number Subunits Comment Organism
3.2.1.39 ? x * 27790, sequence calculation Bacillus amyloliquefaciens

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 beta-1,3-glucanase
-
 Bacillus amyloliquefaciens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.39 Bacillus amyloliquefaciens sequence calculation
-
 5.93

General Information

EC Number General Information Comment Organism
3.2.1.39 additional information phylogenetic tree, sequence comparisons and structure homology modelling, residues Asn52, Ile157 and Val158 form the active site Bacillus amyloliquefaciens