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Literature summary extracted from

  • Lee, S.H.; Lee, Y.E.
    Cloning, expression, and characterization of a thermostable GH51 alpha-L-arabinofuranosidase from Paenibacillus sp. DG-22 (2014), J. Microbiol. Biotechnol., 24, 236-244.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.55 2-mercaptoethanol
-
 Paenibacillus sp.
3.2.1.55 DTT
-
 Paenibacillus sp.
3.2.1.55 EDTA
-
 Paenibacillus sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 gene abfA, DNA and amino acid sequence determination and analysis, sequence comparison, expression of His6-tagged enzyme in Escherichia coli strain M15(pREP4), subcloning in Escherichia coli strain DH5alpha Paenibacillus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.55 Ag+ almost complete inhibition at 1 mM Paenibacillus sp.
3.2.1.55 Ca2+ 83% inhibition at 1 mM, 99.5% at 10 mM Paenibacillus sp.
3.2.1.55 Co2+ almost complete inhibition at 1 mM Paenibacillus sp.
3.2.1.55 Cu2+ almost complete inhibition at 1 mM Paenibacillus sp.
3.2.1.55 Fe2+ 44% inhibition at 1 mM, 95% at 10 mM Paenibacillus sp.
3.2.1.55 Hg2+ complete inhibition at 1 mM Paenibacillus sp.
3.2.1.55 K+ 66% inhibition at 1 mM, 95% at 10 mM Paenibacillus sp.
3.2.1.55 Mg2+ 56% inhibition at 1 mM, 70% at 10 mM Paenibacillus sp.
3.2.1.55 Mn2+ no inhibition at 1 mM, 44% at 10 mM Paenibacillus sp.
3.2.1.55 Na+ slight inhibition at 1-10 mM Paenibacillus sp.
3.2.1.55 Rb+ 6% inhibition at 1 mM, 30% at 10 mM Paenibacillus sp.
3.2.1.55 SDS 71% inhibition at 1 mM, 96% at 10 mM Paenibacillus sp.
3.2.1.55 Zn2+ 96% inhibition at 1 mM, almost complete at 10 mM Paenibacillus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 3.5
-
 4-nitrophenyl-alpha-L-arabinofuranoside recombinant His6-tagged enzyme, pH 6.0, 60°C Paenibacillus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.55 additional information no effect on activity with Mn2+, Na+, and Rb+ Paenibacillus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 56520
-
 4 * 56520, sequence calculation, 4 * 57000, recombinant His-tagged enzyme, SDS-PAGE Paenibacillus sp.
3.2.1.55 57000
-
 4 * 56520, sequence calculation, 4 * 57000, recombinant His-tagged enzyme, SDS-PAGE Paenibacillus sp.
3.2.1.55 221500
-
 recombinant His-tagged enzyme, gel filtration Paenibacillus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.55 additional information Paenibacillus sp. the enzyme catalyzes the hydrolysis of alpha-linked L-arabinofuranosyl residues at the nonreducing ends of arabinose-containing hemicelluloses such as arabinoxylans, arabinans, and arabinogalactans ?
-
 ?
3.2.1.55 additional information Paenibacillus sp. DG-22 the enzyme catalyzes the hydrolysis of alpha-linked L-arabinofuranosyl residues at the nonreducing ends of arabinose-containing hemicelluloses such as arabinoxylans, arabinans, and arabinogalactans ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Paenibacillus sp. A0A075EDT8 gene abfA
-
3.2.1.55 Paenibacillus sp. DG-22 A0A075EDT8 gene abfA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55 recombinant His6-tagged enzyme 7.4fold from Escherichia coli by nickel affinity chromatography to homogeneity Paenibacillus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.55 96.7
-
 purified recombinant His6-tagged enzyme, pH 6.0, 60°C Paenibacillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 4-methylumbelliferyl alpha-L-arabinofuranoside + H2O
-
 Paenibacillus sp. 4-methylumbelliferol + alpha-L-arabinofuranose
-
 ?
3.2.1.55 4-methylumbelliferyl alpha-L-arabinofuranoside + H2O
-
 Paenibacillus sp. DG-22 4-methylumbelliferol + alpha-L-arabinofuranose
-
 ?
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Paenibacillus sp. 4-nitrophenol + L-arabinofuranose
-
 ?
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Paenibacillus sp. DG-22 4-nitrophenol + L-arabinofuranose
-
 ?
3.2.1.55 additional information the enzyme catalyzes the hydrolysis of alpha-linked L-arabinofuranosyl residues at the nonreducing ends of arabinose-containing hemicelluloses such as arabinoxylans, arabinans, and arabinogalactans Paenibacillus sp. ?
-
 ?
3.2.1.55 additional information substrate specificity, overview. Poor activity with p-nitrophenyl beta-D-xylopyranoside Paenibacillus sp. ?
-
 ?
3.2.1.55 additional information the enzyme catalyzes the hydrolysis of alpha-linked L-arabinofuranosyl residues at the nonreducing ends of arabinose-containing hemicelluloses such as arabinoxylans, arabinans, and arabinogalactans Paenibacillus sp. DG-22 ?
-
 ?
3.2.1.55 additional information substrate specificity, overview. Poor activity with p-nitrophenyl beta-D-xylopyranoside Paenibacillus sp. DG-22 ?
-
 ?
3.2.1.55 oat spelt arabinoxylan + H2O
-
 Paenibacillus sp. ?
-
 ?
3.2.1.55 oat spelt arabinoxylan + H2O
-
 Paenibacillus sp. DG-22 ?
-
 ?
3.2.1.55 wheat L-arabinoxylan + H2O
-
 Paenibacillus sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.55 tetramer 4 * 56520, sequence calculation, 4 * 57000, recombinant His-tagged enzyme, SDS-PAGE Paenibacillus sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 AFase
-
 Paenibacillus sp.
3.2.1.55 alpha-L-arabinofuranosidase
-
 Paenibacillus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 60
-
-
 Paenibacillus sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.55 35 75 activity range, profile overview Paenibacillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 6
-
-
 Paenibacillus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.55 4 10 activity range, profile overview Paenibacillus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.55 6 10 purified recombinant enzyme, over 80% activity remaining Paenibacillus sp.

General Information

EC Number General Information Comment Organism
3.2.1.55 evolution the enzyme belongs to the family 51 glycoside hydrolase, GH51 Paenibacillus sp.
3.2.1.55 metabolism the presence of L-arabinofuranoside side chains restricts the action of endo-acting xylanases. Therefore, the AFases are the most important accessory enzymes involved in synergy with other hemicellulases for the complete hydrolysis of hemicelluloses Paenibacillus sp.
3.2.1.55 additional information the recombinant enzyme shows a synergistic effect in combination with endoxylanase on the degradation of oat spelt xylan and wheat arabinoxylan Paenibacillus sp.