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Literature summary extracted from
- Engineering the cellular protein secretory pathway for enhancement of recombinant tissue plasminogen activator expression in Chinese hamster ovary cells: effects of CERT and XBP1s genes (2013), J. Microbiol. Biotechnol., 23, 1116-1122.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.4.21.68 | medicine | recombinant human t-PA is extensively used as a therapeutic agent for the treatment of thrombotic diseases owing to its greater safety and efficiency compared with other plasminogen activators like urokinase and streptokinase. CERTS132A-based secretion engineering can be an effective strategy for enhancing recombinant enzyme production in CHO cells | Homo sapiens |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.21.68 | recombinant overexpression of the enzyme in CHO ATCC CRL-9606 cells and secretion to the cell culture medium, co-expression with CERT S132A, a mutant form of CERT that is resistant to phosphorylation, and XBP1s, method optimization and evaluation, overview. Overexpression of CERT S132A increases the specific productivity of t-PA-producing CHO cells up to 35%, while the heterologous expression of XBP1s does not affect the t-PA expression rate | Homo sapiens |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.21.68 | extracellular | - |
Homo sapiens | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.68 | 68000 | - |
x * 68000, recombinant enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.68 | plasminogen + H2O | Homo sapiens | activation | plasmin + ? | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.68 | Homo sapiens | P00750 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.68 | plasminogen + H2O | activation | Homo sapiens | plasmin + ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.21.68 | ? | x * 68000, recombinant enzyme, SDS-PAGE | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.68 | t-PA | - |
Homo sapiens |
| 3.4.21.68 | Tissue plasminogen activator | - |
Homo sapiens |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.4.21.68 | physiological function | the human tissue plasminogen activator is a serine protease known as the key component of the fibrinolytic system. t-PA converts the proenzyme plasminogen to plasmin, which in turn mediates the degradation of fibrin | Homo sapiens |
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