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Literature summary extracted from
- Cloning, high-level expression, purification, and properties of a novel endo-beta-1,4-mannanase from Bacillus subtilis G1 in Pichia pastoris (2012), J. Microbiol. Biotechnol., 22, 331-338.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.2.1.78 | agriculture | the recombinant enzyme can be an additive in the feed for monogastric animals | Bacillus subtilis |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.78 | gene man5A, DNA and amino acid sequence determination and analysis, recombinant expression in Pichia pastoris strain GS115 | Bacillus subtilis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.78 | acetone | - |
Bacillus subtilis |  |
| 3.2.1.78 | Co2+ | - |
Bacillus subtilis | |
| 3.2.1.78 | EDTA | - |
Bacillus subtilis | |
| 3.2.1.78 | ethanol | - |
Bacillus subtilis | |
| 3.2.1.78 | Hg2+ | - |
Bacillus subtilis | |
| 3.2.1.78 | methanol | - |
Bacillus subtilis | |
| 3.2.1.78 | n-butanol | complete inhibition at 20-30% v/v | Bacillus subtilis | |
| 3.2.1.78 | Pb2+ | - |
Bacillus subtilis | |
| 3.2.1.78 | SDS | complete inhibition at 0.25-1.0% w/v | Bacillus subtilis | |
| 3.2.1.78 | Triton X-100 | - |
Bacillus subtilis | |
| 3.2.1.78 | Tween 20 | - |
Bacillus subtilis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.78 | 41000 | - |
x * 41000, recombinant enzyme, SDS-PAGE | Bacillus subtilis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.78 | Bacillus subtilis | - |
gene manA | - |
| 3.2.1.78 | Bacillus subtilis G1 | - |
gene manA | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.78 | recombinant enzyme from Pichia pastoris strain GS115 | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.78 | 2718 | - |
purified recombinant enzyme, pH 6.5, 45°C | Bacillus subtilis |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.78 | ? | x * 41000, recombinant enzyme, SDS-PAGE | Bacillus subtilis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.78 | endo-beta-1,4-mannanase | - |
Bacillus subtilis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.78 | 45 | - |
recombinant enzyme | Bacillus subtilis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.78 | 50 | - |
purified recombinant enzyme, pH 5.0-9.0, 8 h, stable at | Bacillus subtilis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.78 | 6.5 | - |
recombinant enzyme | Bacillus subtilis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.78 | 5 | 9 | purified recombinant enzyme, 50°C, 8 h, stable at | Bacillus subtilis |
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