Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chuankhayan, P.; Kao, T.T.; Lin, C.C.; Guan, H.H.; Nakagawa, A.; Fu, T.F.; Chen, C.J.
    Structural insights into the hydrolysis and polymorphism of methotrexate polyglutamate by zebrafish gamma-glutamyl hydrolase (2013), J. Med. Chem., 56, 7625-7635.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.19.9 wild-type and mutants C108A and H218N in complex with substrate methotrexate pentaglutamate and product methotrexate glutamate, to 1.9 to 2.4 A resolution. The side chain of residue Phe20 and the 6-methylpterin ring of methotrexate pentaglutamate invoke pi-pi interactions to promote distinct concerted conformational alterations involving about 90° rotations in the complexes with the C108A and H218N mutant proteins Danio rerio

Protein Variants

EC Number Protein Variants Comment Organism
3.4.19.9 C108A complete loss of hydrolytic activity Danio rerio
3.4.19.9 F20A about 40% decrease in hyrolytic activity Danio rerio
3.4.19.9 F20A/C108A complete loss of hydrolytic activity Danio rerio
3.4.19.9 F20R about 40% increase in hyrolytic activity Danio rerio
3.4.19.9 F20R/C108A complete loss of hydrolytic activity Danio rerio
3.4.19.9 H218N complete loss of hydrolytic activity. Residue His218 alone suffices to catalyze the hydrolysis of the gamma-glutamate bond in gamma-glutamyl hydrolase Danio rerio

Organism

EC Number Organism UniProt Comment Textmining
3.4.19.9 Danio rerio Q6NY42
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.19.9 methotrexate pentaglutamate + H2O
-
 Danio rerio methotrexate glutamate + tetra-gamma-L-glutamate
-
 ?