BRENDA - Enzyme Database

Enzymatic and transcriptional regulation of the cytoplasmic acetyl-CoA hydrolase ACOT12

Horibata, Y.; Ando, H.; Itoh, M.; Sugimoto, H.; J. Lipid Res. 54, 2049-2059 (2013)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.2.1
1,2-dimyristoylphosphatidylglycerol
activates mutant form lacking START domain
Mus musculus
3.1.2.1
1-oleoyl-2-lysophosphatidylglycerol
activates mutant form lacking START domain
Mus musculus
3.1.2.1
Dopa
activates mutant form lacking START domain
Mus musculus
3.1.2.1
phosphatidic acid
activates mutant form lacking START domain
Mus musculus
Cloned(Commentary)
EC Number
Commentary
Organism
3.1.2.1
expressed in Escherichia coli as a His-tagged fusion protein
Mus musculus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.1.2.1
additional information
mutant form of ACOT12 lacking the START domain is not inhibited by lipids. Mutant forms both dimers and tetramers whereas wild-type forms only tetramers after addition of ATP
Mus musculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.2.1
1,2-dimyristoyl phosphatidylglycerol
-
Mus musculus
3.1.2.1
1-oleoyl-2-lysophosphatidic acid
-
Mus musculus
3.1.2.1
Dopa
-
Mus musculus
3.1.2.1
phosphatidic acid
-
Mus musculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.1
0.33
-
acetoacetyl-CoA
pH 8, 37°C, Vmax: 30 micromol/min/mg, wild-type
Mus musculus
3.1.2.1
0.36
-
acetyl-CoA
pH 8, 37°C, Vmax: 125 micromol/min/mg, wild-type; pH 8, 37°C, Vmax: 23.4 micromol/min/mg, mutant form lacking START domain
Mus musculus
3.1.2.1
0.41
-
butyryl-CoA
pH 8, 37°C, Vmax: 44 micromol/min/mg, wild-type
Mus musculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.2.1
cytosol
-
Mus musculus
5829
-
3.1.2.1
cytosol
-
Rattus norvegicus
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.1
77000
-
4 * 77000 kDa, 45% of wild-type form active tetramers. 55.7% of mutant form lacking START domain form a mixture of active tetramers (31%) and dimers 23.9%
Mus musculus
3.1.2.1
88000
-
gel filtration, mutant form lacking START domain, pre-incubation with ATP, dimer
Mus musculus
3.1.2.1
182000
-
gel filtration, mutant form lacking START domain, pre-incubation with ATP, tetramer
Mus musculus
3.1.2.1
322000
-
gel filtration, wild-type, pre-incubation with ATP, tetramer
Mus musculus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.2.1
Mus musculus
Q9DBK0
-
-
3.1.2.1
Rattus norvegicus
Q99NB7
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.2.1
using Ni-NTA chromatography
Mus musculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.2.1
liver
-
Mus musculus
-
3.1.2.1
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.2.1
15
-
pH 8, 37°C, mutant form lacking START domain
Mus musculus
3.1.2.1
74.4
-
pH 8, 37°C, wild-type
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.1
acetoacetyl-CoA + H2O
-
732297
Mus musculus
CoA + acetoacetate
-
-
-
?
3.1.2.1
acetyl-CoA + H2O
-
732297
Mus musculus
CoA + acetate
-
-
-
?
3.1.2.1
butyryl-CoA + H2O
-
732297
Mus musculus
CoA + butanoate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.2.1
dimer
55.7% of mutant form lacking START domain form a mixture of active tetramers (31%) and dimers (23.9%)
Mus musculus
3.1.2.1
tetramer
4 * 77000 kDa, 45% of wild-type form active tetramers. 55.7% of mutant form lacking START domain form a mixture of active tetramers (31%) and dimers 23.9%
Mus musculus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.2.1
37
-
assay at
Mus musculus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.1.2.1
1.99
-
acetyl-CoA
pH 8, 37°C, mutant form lacking START domain
Mus musculus
3.1.2.1
7.26
-
acetyl-CoA
pH 8, 37°C, wild-type
Mus musculus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.2.1
8
-
assay at
Mus musculus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.2.1
1,2-dimyristoylphosphatidylglycerol
activates mutant form lacking START domain
Mus musculus
3.1.2.1
1-oleoyl-2-lysophosphatidylglycerol
activates mutant form lacking START domain
Mus musculus
3.1.2.1
Dopa
activates mutant form lacking START domain
Mus musculus
3.1.2.1
phosphatidic acid
activates mutant form lacking START domain
Mus musculus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.2.1
expressed in Escherichia coli as a His-tagged fusion protein
Mus musculus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.1.2.1
additional information
mutant form of ACOT12 lacking the START domain is not inhibited by lipids. Mutant forms both dimers and tetramers whereas wild-type forms only tetramers after addition of ATP
Mus musculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.2.1
1,2-dimyristoyl phosphatidylglycerol
-
Mus musculus
3.1.2.1
1-oleoyl-2-lysophosphatidic acid
-
Mus musculus
3.1.2.1
Dopa
-
Mus musculus
3.1.2.1
phosphatidic acid
-
Mus musculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.1
0.33
-
acetoacetyl-CoA
pH 8, 37°C, Vmax: 30 micromol/min/mg, wild-type
Mus musculus
3.1.2.1
0.36
-
acetyl-CoA
pH 8, 37°C, Vmax: 125 micromol/min/mg, wild-type; pH 8, 37°C, Vmax: 23.4 micromol/min/mg, mutant form lacking START domain
Mus musculus
3.1.2.1
0.41
-
butyryl-CoA
pH 8, 37°C, Vmax: 44 micromol/min/mg, wild-type
Mus musculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.2.1
cytosol
-
Mus musculus
5829
-
3.1.2.1
cytosol
-
Rattus norvegicus
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.1
77000
-
4 * 77000 kDa, 45% of wild-type form active tetramers. 55.7% of mutant form lacking START domain form a mixture of active tetramers (31%) and dimers 23.9%
Mus musculus
3.1.2.1
88000
-
gel filtration, mutant form lacking START domain, pre-incubation with ATP, dimer
Mus musculus
3.1.2.1
182000
-
gel filtration, mutant form lacking START domain, pre-incubation with ATP, tetramer
Mus musculus
3.1.2.1
322000
-
gel filtration, wild-type, pre-incubation with ATP, tetramer
Mus musculus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.2.1
using Ni-NTA chromatography
Mus musculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.2.1
liver
-
Mus musculus
-
3.1.2.1
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.2.1
15
-
pH 8, 37°C, mutant form lacking START domain
Mus musculus
3.1.2.1
74.4
-
pH 8, 37°C, wild-type
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.1
acetoacetyl-CoA + H2O
-
732297
Mus musculus
CoA + acetoacetate
-
-
-
?
3.1.2.1
acetyl-CoA + H2O
-
732297
Mus musculus
CoA + acetate
-
-
-
?
3.1.2.1
butyryl-CoA + H2O
-
732297
Mus musculus
CoA + butanoate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.2.1
dimer
55.7% of mutant form lacking START domain form a mixture of active tetramers (31%) and dimers (23.9%)
Mus musculus
3.1.2.1
tetramer
4 * 77000 kDa, 45% of wild-type form active tetramers. 55.7% of mutant form lacking START domain form a mixture of active tetramers (31%) and dimers 23.9%
Mus musculus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.2.1
37
-
assay at
Mus musculus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.1.2.1
1.99
-
acetyl-CoA
pH 8, 37°C, mutant form lacking START domain
Mus musculus
3.1.2.1
7.26
-
acetyl-CoA
pH 8, 37°C, wild-type
Mus musculus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.2.1
8
-
assay at
Mus musculus
Expression
EC Number
Organism
Commentary
Expression
3.1.2.1
Rattus norvegicus
ACOT12 mRNA and protein levels in rat primary hepatocytes decrease following treatment with insulin
down
Expression (protein specific)
EC Number
Organism
Commentary
Expression
3.1.2.1
Rattus norvegicus
ACOT12 mRNA and protein levels in rat primary hepatocytes decrease following treatment with insulin
down