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Literature summary extracted from

  • Han, S.; Cohen, D.E.
    Functional characterization of thioesterase superfamily member 1/Acyl-CoA thioesterase 11: implications for metabolic regulation (2012), J. Lipid Res., 53, 2620-2631.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.2.20 ATP
-
 Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.20 expressed as a GST-fusion protein in Escherichia coli Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.2.20 additional information thioesterase domain alone of Them1 is sufficient to yield active recombinant protein, START domain is required for optimal enzyme activity Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.20 ADP
-
 Mus musculus
3.1.2.20 CoASH
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.20 0.0075
-
 palmitoyl-CoA pH 7.5, 37°C Mus musculus
3.1.2.20 0.0079
-
 lauroyl-CoA pH 7.5, 37°C Mus musculus
3.1.2.20 0.0081
-
 myristoyl-CoA pH 7.5, 37°C Mus musculus
3.1.2.20 0.0093
-
 linoleoyl-CoA pH 7.5, 37°C Mus musculus
3.1.2.20 0.0128
-
 oleoyl-CoA pH 7.5, 37°C Mus musculus
3.1.2.20 0.0133
-
 Arachidonoyl-CoA pH 7.5, 37°C Mus musculus
3.1.2.20 0.0136
-
 palmitoleoyl-CoA pH 7.5, 37°C Mus musculus
3.1.2.20 0.0232
-
 malonyl-CoA pH 7.5, 37°C Mus musculus
3.1.2.20 0.0279
-
 stearoyl-CoA pH 7.5, 37°C Mus musculus
3.1.2.20 0.0479
-
 DL-3-hydroxybutyryl-CoA pH 7.5, 37°C Mus musculus
3.1.2.20 0.0481
-
 acetyl-CoA pH 7.5, 37°C Mus musculus
3.1.2.20 0.0498
-
 phenylacetyl-CoA pH 7.5, 37°C Mus musculus
3.1.2.20 0.0682
-
 2-butenoyl-CoA pH 7.5, 37°C Mus musculus
3.1.2.20 0.0868
-
 DL-3-hydroxy-3-methylglutaryl-CoA pH 7.5, 37°C Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.20 cytoplasm concentrated to the greatest extent in liver Mus musculus 5737
-
3.1.2.20 microsome concentrated to the greatest extent in brown adipose tissue Mus musculus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.20 112000
-
 FPLC Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.20 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.20 affinity chromatography Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.20 brown adipose tissue
-
 Mus musculus
-
3.1.2.20 liver
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.20 2-butenoyl-CoA + H2O
-
 Mus musculus CoA + 2-butenoate
-
 ?
3.1.2.20 acetyl-CoA + H2O
-
 Mus musculus CoA + acetate
-
 ?
3.1.2.20 arachidonoyl-CoA + H2O
-
 Mus musculus CoA + arachidonate
-
 ?
3.1.2.20 DL-3-hydroxy-3-methylglutaryl-CoA + H2O
-
 Mus musculus CoA + 3-hydroxy-3-methylglutarate
-
 ?
3.1.2.20 DL-3-hydroxybutyryl-CoA + H2O
-
 Mus musculus ?
-
 ?
3.1.2.20 lauroyl-CoA + H2O
-
 Mus musculus CoA + lauroate
-
 ?
3.1.2.20 linoleoyl-CoA + H2O
-
 Mus musculus CoA + linoleate
-
 ?
3.1.2.20 malonyl-CoA + H2O
-
 Mus musculus CoA + malonate
-
 ?
3.1.2.20 myristoyl-CoA + H2O
-
 Mus musculus CoA + myristate
-
 ?
3.1.2.20 oleoyl-CoA + H2O
-
 Mus musculus CoA + oleate
-
 ?
3.1.2.20 palmitoleoyl-CoA + H2O
-
 Mus musculus CoA + palmitoleoate
-
 ?
3.1.2.20 palmitoyl-CoA + H2O
-
 Mus musculus CoA + palmitate
-
 ?
3.1.2.20 phenylacetyl-CoA + H2O
-
 Mus musculus CoA + phenylacetate
-
 ?
3.1.2.20 stearoyl-CoA + H2O
-
 Mus musculus CoA + stearate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.20 dimer
-
 Mus musculus

Synonyms

EC Number Synonyms Comment Organism
3.1.2.20 acyl-CoA thioesterase 11
-
 Mus musculus
3.1.2.20 StarD14
-
 Mus musculus
3.1.2.20 Them1
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.20 37
-
 assay at Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.2.20 1.2
-
 Arachidonoyl-CoA pH 7.5, 37°C Mus musculus
3.1.2.20 2.3
-
 stearoyl-CoA pH 7.5, 37°C Mus musculus
3.1.2.20 2.7
-
 linoleoyl-CoA pH 7.5, 37°C Mus musculus
3.1.2.20 3.2
-
 oleoyl-CoA pH 7.5, 37°C Mus musculus
3.1.2.20 3.2
-
 phenylacetyl-CoA pH 7.5, 37°C Mus musculus
3.1.2.20 4.7
-
 myristoyl-CoA pH 7.5, 37°C Mus musculus
3.1.2.20 4.7
-
 palmitoleoyl-CoA pH 7.5, 37°C Mus musculus
3.1.2.20 5.3
-
 lauroyl-CoA pH 7.5, 37°C Mus musculus
3.1.2.20 8.5
-
 palmitoyl-CoA pH 7.5, 37°C Mus musculus
3.1.2.20 9.6
-
 malonyl-CoA pH 7.5, 37°C Mus musculus
3.1.2.20 11
-
 2-butenoyl-CoA pH 7.5, 37°C Mus musculus
3.1.2.20 12
-
 DL-3-hydroxy-3-methylglutaryl-CoA pH 7.5, 37°C Mus musculus
3.1.2.20 13
-
 DL-3-hydroxybutyryl-CoA pH 7.5, 37°C Mus musculus
3.1.2.20 24
-
 acetyl-CoA pH 7.5, 37°C Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.20 7.5
-
 assay at Mus musculus