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Literature summary extracted from
- Purification and characterization of a prolyl endopeptidase isolated from Aspergillus oryzae (2014), J. Ind. Microbiol. Biotechnol., 41, 49-55.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.26 | Al3+ | 65% residual activity at 10 mM | Aspergillus oryzae |  |
| 3.4.21.26 | Aprotinin | 89% residual activity at 1 mM | Aspergillus oryzae | |
| 3.4.21.26 | Cu2+ | 12% residual activity at 1 mM | Aspergillus oryzae | |
| 3.4.21.26 | EDTA | 37.3% residual activity at 10 mM | Aspergillus oryzae | |
| 3.4.21.26 | Fe2+ | 55% residual activity at 10 mM | Aspergillus oryzae | |
| 3.4.21.26 | leupeptin | 86% residual activity at 1 mM | Aspergillus oryzae | |
| 3.4.21.26 | Mg2+ | 97% residual activity at 10 mM | Aspergillus oryzae | |
| 3.4.21.26 | Mn2+ | 30% residual activity at 10 mM | Aspergillus oryzae | |
| 3.4.21.26 | phenylmethylsulfonyl fluoride | 97% residual activity at 1 mM | Aspergillus oryzae | |
| 3.4.21.26 | Zn2+ | 91% residual activity at 10 mM | Aspergillus oryzae | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.26 | 0.18 | - |
L-Ala-L-Ala-L-Pro-4-nitroanilide | at pH 5.0 and 35°C | Aspergillus oryzae | |
| 3.4.21.26 | 0.26 | - |
Z-Gly-L-Pro-4-nitroanilide | at pH 5.0 and 35°C | Aspergillus oryzae | |
| 3.4.21.26 | 0.37 | - |
Z-L-Ala-L-Ala-L-Ala-L-Pro-4-nitroanilide | at pH 5.0 and 35°C | Aspergillus oryzae | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.26 | Ca2+ | 100% activity at 10 mM | Aspergillus oryzae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.26 | 60000 | - |
x * 60000, SDS-PAGE | Aspergillus oryzae |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.26 | casein + H2O | Aspergillus oryzae | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.26 | Aspergillus oryzae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.26 | ammonium sulfate precipitation, HiTrap DEAE column chromatography, and HiTrap Q column chromatography | Aspergillus oryzae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.26 | 50 | - |
crude extract, at pH 5.0 and 35°C | Aspergillus oryzae |
| 3.4.21.26 | 3650 | - |
after 73fold purification, at pH 5.0 and 35°C | Aspergillus oryzae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.26 | casein + H2O | - |
Aspergillus oryzae | ? | - |
? | |
| 3.4.21.26 | L-Ala-L-Ala-L-Pro-4-nitroanilide + H2O | - |
Aspergillus oryzae | L-Ala-L-Ala-L-Pro + 4-nitroaniline | - |
? | |
| 3.4.21.26 | Z-Gly-L-Pro-4-nitroanilide + H2O | - |
Aspergillus oryzae | Z-Gly-L-Pro + 4-nitroaniline | - |
? | |
| 3.4.21.26 | Z-L-Ala-L-Ala-L-Ala-L-Pro-4-nitroanilide + H2O | - |
Aspergillus oryzae | Z-L-Ala-L-Ala-L-Ala-L-Pro + 4-nitroaniline | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.21.26 | ? | x * 60000, SDS-PAGE | Aspergillus oryzae |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.26 | POP | - |
Aspergillus oryzae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.26 | 30 | - |
- |
Aspergillus oryzae |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.26 | 55 | - |
the residual activity after 30 min incubation at 55°C is higher than 80% | Aspergillus oryzae |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.26 | 75 | - |
Z-L-Ala-L-Ala-L-Ala-L-Pro-4-nitroanilide | at pH 5.0 and 35°C | Aspergillus oryzae | |
| 3.4.21.26 | 107.4 | - |
L-Ala-L-Ala-L-Pro-4-nitroanilide | at pH 5.0 and 35°C | Aspergillus oryzae | |
| 3.4.21.26 | 240 | - |
Z-Gly-L-Pro-4-nitroanilide | at pH 5.0 and 35°C | Aspergillus oryzae | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.26 | 4 | - |
- |
Aspergillus oryzae |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.26 | 3 | 5 | more than 70% of the initial activity remains after incubation of the enzyme for 30 min at 35°C between pH 3.0 and 5.0 | Aspergillus oryzae |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.26 | 24 | - |
Z-Gly-L-Pro-4-nitroanilide | at pH 5.0 and 35°C | Aspergillus oryzae | |
| 3.4.21.26 | 202.7 | - |
Z-L-Ala-L-Ala-L-Ala-L-Pro-4-nitroanilide | at pH 5.0 and 35°C | Aspergillus oryzae | |
| 3.4.21.26 | 596.7 | - |
L-Ala-L-Ala-L-Pro-4-nitroanilide | at pH 5.0 and 35°C | Aspergillus oryzae | |
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