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Literature summary extracted from

  • Lee, C.C.; Kibblewhite, R.E.; Wagschal, K.; Li, R.; Robertson, G.H.; Orts, W.J.
    Isolation and characterization of a novel GH67 alpha-glucuronidase from a mixed culture (2012), J. Ind. Microbiol. Biotechnol., 39, 1245-1251.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.131 xylanase when the enzyme is used in combination with xylanase, a large stimulation in activity occurs uncultured bacterium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.131 expressed in Escherichia coli BL21(DE3)pLysE cells uncultured bacterium
3.2.1.139 gene deg75-AG, DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)pLysE uncultured bacterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.131 Ca2+ Ca2+ addition results in a slight activity decrease (86.3%) uncultured bacterium
3.2.1.139 Ca2+ 14% inhibition at 1 mM uncultured bacterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.131 Mn2+ the presence of manganese produces higher activity (117.4%) uncultured bacterium
3.2.1.131 additional information enzyme activity is not dramatically impacted by Co2+, Cu2+, Fe2+, Li+, Mg2+, and Zn2+ at 1 mM uncultured bacterium
3.2.1.139 Mn2+ 17% activation at 1 mM uncultured bacterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.131 84500
-
 x * 84500, calculated from amino acid sequence uncultured bacterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.131 birchwood xylan + H2O uncultured bacterium
-
 4-O-methyl-alpha-D-glucuronic acid + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.131 uncultured bacterium I2E7L0
-
-
3.2.1.139 uncultured bacterium
-
 gene deg75-AG
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.131 HisTrap HP column chromatography uncultured bacterium
3.2.1.139 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLysE by immobilized metal affinity chromatography and gell filtration uncultured bacterium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.139 11.7
-
 purified recombinant enzyme, pH 8.0, 45°C uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.131 birchwood xylan + H2O
-
 uncultured bacterium 4-O-methyl-alpha-D-glucuronic acid + ?
-
 ?
3.2.1.139 birchwood xylan + H2O
-
 uncultured bacterium ? release of reduced sugar ?
3.2.1.139 additional information the enzyme functions synergistically with xylanase in the hydrolysis of xylan substrate. The main products of the hydrolysis reaction are xylotriose, xylotetraose, and xylopentaose uncultured bacterium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.131 ? x * 84500, calculated from amino acid sequence uncultured bacterium

Synonyms

EC Number Synonyms Comment Organism
3.2.1.131 alpha-glucuronidase
-
 uncultured bacterium
3.2.1.131 DEG75-AG
-
 uncultured bacterium
3.2.1.139 DEG75-AG
-
 uncultured bacterium
3.2.1.139 GH67 alpha-glucuronidase
-
 uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.131 45
-
-
 uncultured bacterium
3.2.1.139 45
-
-
 uncultured bacterium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.139 30 60 activity range, profile overview uncultured bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.131 40 60 after 2 h at 40 and 50°C, the enzyme retains most of the initial activity. At 60 °C, half of the activity is lost at 30 min, and no activity remains after 1 h uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.131 7 8
-
 uncultured bacterium
3.2.1.139 7 8
-
 uncultured bacterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.131 6 9 there is a dramatic decrease in enzyme activity at pH 6.0 and pH 9.0, and there is little activity at pH 4.0 and pH 5.0 uncultured bacterium
3.2.1.139 5.5 9.5 activity range, profile overview uncultured bacterium

General Information

EC Number General Information Comment Organism
3.2.1.139 additional information the enzyme protein sequence contains two highly conserved amino acids, D43 and E462, that are implicated as critical catalytic residues uncultured bacterium