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Literature summary extracted from

  • Culleton, H.; Mckie, V.; De Vries, R.
    Overexpression, purification and characterisation of homologous alpha-L-arabinofuranosidase and endo-1,4-beta-D-glucanase in Aspergillus vadensis (2014), J. Ind. Microbiol. Biotechnol., 14, 1697-1708.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 gene abfB, DNA and amino acid sequence determination and analysis, recombinant extracellular expression of His-tagged enzyme in Aspergillus vadensis strain CBS 113365 using gpdA promoter from Aspergillus vadensis, method development and evaluation, overview Aspergillus vadensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.55 extracellular the enzyme is secreted Aspergillus vadensis
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Aspergillus vadensis
-
 gene abfB
-
3.2.1.55 Aspergillus vadensis CBS 137441
-
 gene abfB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55 recombinant His-tagged enzyme 75fold from Aspergillus vadensis strain CBS 113365 culture supernatant Aspergillus vadensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 4-methylumbelliferyl-alpha-L-arabinopyranoside + H2O artificial Aspergillus vadensis 4-methylumbelliferol + alpha-L-arabinopyranose
-
 ?
3.2.1.55 4-methylumbelliferyl-alpha-L-arabinopyranoside + H2O artificial Aspergillus vadensis CBS 137441 4-methylumbelliferol + alpha-L-arabinopyranose
-
 ?
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O best substrate Aspergillus vadensis 4-nitrophenol + L-arabinofuranose
-
 ?
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O best substrate Aspergillus vadensis CBS 137441 4-nitrophenol + L-arabinofuranose
-
 ?
3.2.1.55 debranched sugar beet arabinan + H2O low activity Aspergillus vadensis ?
-
 ?
3.2.1.55 debranched sugar beet arabinan + H2O low activity Aspergillus vadensis CBS 137441 ?
-
 ?
3.2.1.55 sugar beet arabinan + H2O low activity Aspergillus vadensis ?
-
 ?
3.2.1.55 sugar beet arabinan + H2O low activity Aspergillus vadensis CBS 137441 ?
-
 ?
3.2.1.55 wheat flour arabinoxylan + H2O low activity Aspergillus vadensis ?
-
 ?
3.2.1.55 wheat flour arabinoxylan + H2O low activity Aspergillus vadensis CBS 137441 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 alpha-arabinofuranosidase B
-
 Aspergillus vadensis
3.2.1.55 alpha-L-arabinofuranosidase
-
 Aspergillus vadensis
3.2.1.55 AvAbfB
-
 Aspergillus vadensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 60
-
 recombinant enzyme Aspergillus vadensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.55 25 70 activity range, recombinant enzyme, overview Aspergillus vadensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.55 50
-
 purified recombinant His-tagged enzyme, 15 min, stable up to Aspergillus vadensis
3.2.1.55 60
-
 purified recombinant His-tagged enzyme, 15 min, 77% activity remaining Aspergillus vadensis
3.2.1.55 70
-
 purified recombinant His-tagged enzyme, 15 min, inactivation Aspergillus vadensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 3.5
-
 recombinant enzyme Aspergillus vadensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.55 1 6 activity range, recombinant enzyme, overview Aspergillus vadensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.55 2 9 purified recombinant His-tagged enzyme, 40°C, 1 h, completely stable at Aspergillus vadensis

General Information

EC Number General Information Comment Organism
3.2.1.55 evolution the enzyme belongs to glycosyl hydrolase family 54, GH54 Aspergillus vadensis