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Literature summary extracted from

  • Julian, I.; Gandullo, J.; Santos-Silva, L.K.; Diaz, I.; Martinez, M.
    Phylogenetically distant barley legumains have a role in both seed and vegetative tissues (2013), J. Exp. Bot., 64, 2929-2941.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.34 expressed in Pichia pastoris KM71H cells Hordeum vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.34 cystatin CPI-4
-
 Hordeum vulgare

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.34 endoplasmic reticulum
-
 Hordeum vulgare 5783
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.34 41000
-
 x * 41000, recombinant enzyme, SDS-PAGE Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.34 Hordeum vulgare
-
 cultivar Bomi
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.34 ammonium sulfate precipitation Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.34 aleurone
-
 Hordeum vulgare
-
3.4.22.34 embryo
-
 Hordeum vulgare
-
3.4.22.34 endosperm
-
 Hordeum vulgare
-
3.4.22.34 leaf
-
 Hordeum vulgare
-
3.4.22.34 seed
-
 Hordeum vulgare
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.34 Z-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O
-
 Hordeum vulgare Z-Ala-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.34 ? x * 41000, recombinant enzyme, SDS-PAGE Hordeum vulgare

Synonyms

EC Number Synonyms Comment Organism
3.4.22.34 Leg-2 isoform Hordeum vulgare
3.4.22.34 Leg-4 isoform Hordeum vulgare
3.4.22.34 vacuolar processing enzyme
-
 Hordeum vulgare

Expression

EC Number Organism Comment Expression
3.4.22.34 Hordeum vulgare isoform Leg-4 is repressed after abscisic acid exposure down
3.4.22.34 Hordeum vulgare isoform Leg-2 mRNA expression responds in leaves to biotic and abiotic stimuli, such as salicylic acid, jasmonic acid, nitric oxide, abscisic acid, and aphid infestation, and is induced by gibberellic acid in seeds. Isoform Leg-4 responds in leaves to wounding, nitric oxide, and abscisic acid treatments up