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Literature summary extracted from

  • Satou, M.; Nishi, Y.; Hishinuma, A.; Hosoda, H.; Kangawa, K.; Sugimoto, H.
    Identification of activated protein C as a ghrelin endopeptidase in bovine plasma (2015), J. Endocrinol., 224, 61-73.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.69 ProTac a protein C-activating agent Homo sapiens
3.4.21.69 ProTac a protein C-activating agent Bos taurus
3.4.21.69 ProTac a protein C-activating agent isolated from snake venom, administration to mice enhances the production of octanoyl ghrelin(1-15) in circulation Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.69 ProTac a protein C activator isolated from snake venom Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.69 ghrelin + H2O Mus musculus ghrelin is converted into smaller fragments in blood plasma in circulation under thrombotic and inflammatory conditions ?
-
 ?
3.4.21.69 ghrelin + H2O Homo sapiens ghrelin is converted into smaller fragments in blood plasma in circulation under thrombotic and inflammatory conditions ?
-
 ?
3.4.21.69 ghrelin + H2O Bos taurus ghrelin is converted into smaller fragments in blood plasma in circulation under thrombotic and inflammatory conditions ?
-
 ?
3.4.21.69 ghrelin + H2O Mus musculus C57/BL6J ghrelin is converted into smaller fragments in blood plasma in circulation under thrombotic and inflammatory conditions ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.69 Bos taurus
-
-
-
3.4.21.69 Homo sapiens
-
-
-
3.4.21.69 Mus musculus
-
-
-
3.4.21.69 Mus musculus C57/BL6J
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.69 native enzyme from bovine plasma by gel filtration, mannose affinity and Blue Sepharose chromatography Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.69 blood plasma
-
 Mus musculus
-
3.4.21.69 blood plasma
-
 Homo sapiens
-
3.4.21.69 blood plasma
-
 Bos taurus
-
3.4.21.69 commercial preparation from plasma Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.69 57.3
-
 purified native enzyme, amidolytic activity, pH 8.0, 37°C Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.69 Boc-Leu-Ser-Thr-Arg-4-nitroanilide + H2O
-
 Mus musculus Boc-Leu-Ser-Thr-Arg + 4-nitroaniline
-
 ?
3.4.21.69 Boc-Leu-Ser-Thr-Arg-4-nitroanilide + H2O amidolytic activity Bos taurus Boc-Leu-Ser-Thr-Arg + 4-nitroaniline
-
 ?
3.4.21.69 Boc-Leu-Ser-Thr-Arg-4-nitroanilide + H2O
-
 Mus musculus C57/BL6J Boc-Leu-Ser-Thr-Arg + 4-nitroaniline
-
 ?
3.4.21.69 ghrelin + H2O ghrelin is converted into smaller fragments in blood plasma in circulation under thrombotic and inflammatory conditions Mus musculus ?
-
 ?
3.4.21.69 ghrelin + H2O ghrelin is converted into smaller fragments in blood plasma in circulation under thrombotic and inflammatory conditions Homo sapiens ?
-
 ?
3.4.21.69 ghrelin + H2O ghrelin is converted into smaller fragments in blood plasma in circulation under thrombotic and inflammatory conditions Bos taurus ?
-
 ?
3.4.21.69 ghrelin + H2O ghrelin is converted into smaller fragments in blood plasma in circulation under thrombotic and inflammatory conditions Mus musculus C57/BL6J ?
-
 ?
3.4.21.69 additional information both acyl and desacyl ghrelin are hydrolyzed at the peptide bond between Arg15 and Lys16, generating an N-terminal peptide consisting of the first 15 residues Mus musculus ?
-
 ?
3.4.21.69 additional information both acyl and desacyl ghrelin are hydrolyzed at the peptide bond between Arg15 and Lys16, generating an N-terminal peptide consisting of the first 15 residues Homo sapiens ?
-
 ?
3.4.21.69 additional information bovine enzyme preferentially hydrolyzes the major form of octanoylated ghrelin(28), i.e. about twice as much hydrolysis as is observed for a longer acyl chain of decanoylated ghrelin(28). But both acyl and desacyl ghrelin are hydrolyzed at the peptidebond between Arg15 and Lys16, generating an N-terminal peptide consisting of the first 15 residues Bos taurus ?
-
 ?
3.4.21.69 additional information both acyl and desacyl ghrelin are hydrolyzed at the peptide bond between Arg15 and Lys16, generating an N-terminal peptide consisting of the first 15 residues Mus musculus C57/BL6J ?
-
 ?
3.4.21.69 octanoyl-ghrelin + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.69 octanoyl-ghrelin + H2O
-
 Bos taurus ?
-
 ?
3.4.21.69 octanoyl-ghrelin + H2O preferred substrate Mus musculus octanoyl ghrelin(1-15) + ?
-
 ?
3.4.21.69 octanoyl-ghrelin + H2O preferred substrate Mus musculus C57/BL6J octanoyl ghrelin(1-15) + ?
-
 ?
3.4.21.69 octanoyl-truncated ghrelin + H2O preferred substrate, synthetic human substrate, octanoyl-truncated ghrelin(1-15) activates GHSR1a-dependent signaling similar to the full-length peptide Homo sapiens ?
-
 ?
3.4.21.69 octanoyl-truncated ghrelin + H2O preferred substrate, synthetic human substrate, octanoyl-truncated ghrelin(1-15) activates GHSR1a-dependent signaling similar to the full-length peptide Bos taurus ?
-
 ?
3.4.21.69 octanoyl-truncated ghrelin + H2O octanoyl-truncated ghrelin(1-15) activates GHSR1a-dependent signaling similar to the full-length peptide Mus musculus ghrelin(1-15) + ?
-
 ?
3.4.21.69 octanoyl-truncated ghrelin + H2O octanoyl-truncated ghrelin(1-15) activates GHSR1a-dependent signaling similar to the full-length peptide Mus musculus C57/BL6J ghrelin(1-15) + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.69 Activated protein C
-
 Mus musculus
3.4.21.69 Activated protein C
-
 Homo sapiens
3.4.21.69 Activated protein C
-
 Bos taurus
3.4.21.69 anticoagulant serine protease-activated protein C
-
 Mus musculus
3.4.21.69 anticoagulant serine protease-activated protein C
-
 Homo sapiens
3.4.21.69 anticoagulant serine protease-activated protein C
-
 Bos taurus
3.4.21.69 APC
-
 Mus musculus
3.4.21.69 APC
-
 Homo sapiens
3.4.21.69 APC
-
 Bos taurus
3.4.21.69 ghrelin endopeptidase
-
 Mus musculus
3.4.21.69 ghrelin endopeptidase
-
 Homo sapiens
3.4.21.69 ghrelin endopeptidase
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.69 22
-
 assay at room temperature Mus musculus
3.4.21.69 37
-
 assay at Homo sapiens
3.4.21.69 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.69 8
-
 assay at Mus musculus
3.4.21.69 8
-
 assay at Bos taurus

General Information

EC Number General Information Comment Organism
3.4.21.69 additional information enzyme peptide mass fingerprinting analysis Mus musculus
3.4.21.69 physiological function the enzyme is a major regulator of blood coagulation by inactivating factors Va and VIIIa. O-ghrelin(15) has no effect Mus musculus
3.4.21.69 physiological function the enzyme is a major regulator of blood coagulation by inactivating factors Va and VIIIa. O-ghrelin(15) has no effect Homo sapiens
3.4.21.69 physiological function the enzyme is a major regulator of blood coagulation by inactivating factors Va and VIIIa. O-ghrelin(15) has no effect Bos taurus