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Literature summary extracted from
- In vitro production and purification of isochorismate using a two-enzyme cascade (2014), J. Biotechnol., 191, 93-98.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.3.2.13 | expressed in Escherichia coli BL21(DE3) cells | Streptomyces hygroscopicus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.3.2.13 | chorismate + H2O | Streptomyces hygroscopicus | strict preference for chorismate | 3,4-dihydroxycyclohexa-1,5-dienecarboxylate + pyruvate | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.3.2.13 | Streptomyces hygroscopicus | - |
subsp. ascomyceticus | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.3.2.13 | Ni-NTA affinity column chromatography | Streptomyces hygroscopicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.3.2.13 | chorismate + H2O | strict preference for chorismate | Streptomyces hygroscopicus | 3,4-dihydroxycyclohexa-1,5-dienecarboxylate + pyruvate | - |
? | |
| 3.3.2.13 | additional information | isochorismate is not accepted as a substrate | Streptomyces hygroscopicus | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.3.2.13 | fkbO | - |
Streptomyces hygroscopicus |
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Release 2023.1 (January 2023)
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