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Literature summary extracted from

  • Xu, L.; Shen, Y.; Hou, J.; Peng, B.; Tang, H.; Bao, X.
    Secretory pathway engineering enhances secretion of cellobiohydrolase I from Trichoderma reesei in Saccharomyces cerevisiae (2014), J. Biosci. Bioeng., 117, 45-52.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.176 additional information overexpression of the protein disulfide isomerase Sc-PDI1 and the plasma membrane targeting soluble N-ethylmaleimide-sensitive factor attachment protein receptor Sc-SSO1, and disruption of the sorting receptor Sc-VPS10 and a Ca2D/Mn2D ATPase Sc-PMR1, improve respectively the extracellular Tr-Cel7A activities Trichoderma reesei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.176 expressed in Saccharomyces cerevisiae strain CEN.PK102-3A Trichoderma reesei
3.2.1.176 gene cel7AF, recombinant expression of extracellular C-terminally FLAG-tagged enzyme with its native signal peptide in Saccharomyces cerevisiae strain CEN.PK102-3, the enzyme is secreted to the culture medium Trichoderma reesei

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.176 additional information secretory pathway engineering, including protein folding, disulfide bond formation, and protein trafficking and sorting, enhances secretion of cellobiohydrolase I, with its native signal peptide, from Trichoderma reesei in Saccharomyces cerevisiae. Overexpression of the protein disulfide isomerase Sc-PDI1 and the plasma membrane targeting soluble N-ethylmaleimide-sensitive factor attachment protein receptor Sc-SSO1, and disruption of the sorting receptor Sc-VPS10 and a Ca2+/Mn2+ ATPase Sc-PMR1, improve respectively the extracellular Tr-Cel7A activities. The extracellular activities of the quadruple-modified strain using 4-nitrophenyl-beta-D-cellobioside and phosphoric acid swollen cellulose as the substrates, respectively, are 3.9fold and 1.3fold higher than that of the reference cel7AF strain, detailed overview Trichoderma reesei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.176 extracellular
-
 Trichoderma reesei
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.176 phosphoric acid swollen cellulose + H2O Trichoderma reesei
-
 cellobiose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.176 Trichoderma reesei
-
-
-
3.2.1.176 Trichoderma reesei G0RVK1 gene cel7AF
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.176 glycoprotein
-
 Trichoderma reesei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.176 4-methylumbelliferyl beta-D-cellobioside + H2O
-
 Trichoderma reesei 4-methylumbelliferone + beta-D-cellobiose
-
 ?
3.2.1.176 4-nitrophenyl beta-D-cellobioside + H2O
-
 Trichoderma reesei 4-nitrophenol + D-cellobiose
-
 ?
3.2.1.176 phosphoric acid swollen cellulose + H2O
-
 Trichoderma reesei cellobiose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.176 Cel7A
-
 Trichoderma reesei
3.2.1.176 cellobiohydrolase I
-
 Trichoderma reesei
3.2.1.176 Tr-Cel7A
-
 Trichoderma reesei