Literature summary extracted from

McVey, C.E.; Ferreira, M.J.; Correia, B.; Lahiri, S.; de Sanctis, D.; Carrondo, M.A.; Lindley, P.F.; de Sa Nogueira, I.; Soares, C.M.; Bento, I.
The importance of the Abn2 calcium cluster in the endo-1,5-arabinanase activity from Bacillus subtilis (2014), J. Biol. Inorg. Chem., 19, 505-513.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.2.1.99	expressed in Escherichia coli BL21(DE3) pLysS cells	Bacillus subtilis

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
3.2.1.99	vapor diffusion method, using 10 mM NiCl2, 100 mM tris(hydroxymethyl)aminomethane-HCl pH 8.5, and 20% PEG 2000 monomethyl ether	Bacillus subtilis

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.2.1.99	H318Q	inactive, contains Ni2+ instead of Ca2+	Bacillus subtilis

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.2.1.99	EDTA	-	Bacillus subtilis
3.2.1.99	EGTA	-	Bacillus subtilis

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
3.2.1.99	Ca2+	the enzyme contains Ca2+ which also stimulates activity	Bacillus subtilis
3.2.1.99	Mn2+	stimulates activity	Bacillus subtilis
3.2.1.99	Ni2+	stimulates activity	Bacillus subtilis

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.2.1.99	Bacillus subtilis	P42293	-	-
3.2.1.99	Bacillus subtilis 168	P42293	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.2.1.99	HisTrap column chromatography	Bacillus subtilis

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.2.1.99	1,5-alpha-L-arabinan + H2O	-	Bacillus subtilis	oligo-alpha-(1->5)-L-arabinoside + L-arabinose	-	?
3.2.1.99	1,5-alpha-L-arabinan + H2O	-	Bacillus subtilis 168	oligo-alpha-(1->5)-L-arabinoside + L-arabinose	-	?
3.2.1.99	sugar beet arabinan + H2O	-	Bacillus subtilis	oligo-alpha-(1->5)-L-arabinoside + L-arabinose	-	?
3.2.1.99	sugar beet arabinan + H2O	-	Bacillus subtilis 168	oligo-alpha-(1->5)-L-arabinoside + L-arabinose	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
3.2.1.99	Abn2	-	Bacillus subtilis
3.2.1.99	endo-1,5-arabinase	-	Bacillus subtilis

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Release 2023.1 (January 2023)