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Literature summary extracted from

  • Nunes, C.; Bras, J.; Najmudin, S.; Moura, J.; Moura, I.; Carepo, M.
    ArsC3 from Desulfovibrio alaskensis G20, a cation and sulfate-independent highly efficient arsenate reductase (2014), J. Biol. Inorg. Chem., 19, 1277-1285.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.20.4.4 expression in Escherichia coli Oleidesulfovibrio alaskensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.20.4.4 0.006
-
 arsenate pH 7.6, 37°C, presence of 150 mM KCl Oleidesulfovibrio alaskensis
1.20.4.4 0.007
-
 arsenate pH 7.6, 37°C, presence of 50 mM K2SO4 Oleidesulfovibrio alaskensis
1.20.4.4 0.008
-
 arsenate pH 7.6, 37°C, presence of 150 mM NaCl Oleidesulfovibrio alaskensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.20.4.4 K+ nonspecific binding Oleidesulfovibrio alaskensis
1.20.4.4 Na+ nonspecific binding Oleidesulfovibrio alaskensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.20.4.4 16000
-
 1 * 16010, calculated, 1 * 16000, SDS-PAGE, 1 * 16008, ESI-microTOF Oleidesulfovibrio alaskensis
1.20.4.4 16008
-
 1 * 16010, calculated, 1 * 16000, SDS-PAGE, 1 * 16008, ESI-microTOF Oleidesulfovibrio alaskensis
1.20.4.4 16010
-
 1 * 16010, calculated, 1 * 16000, SDS-PAGE, 1 * 16008, ESI-microTOF Oleidesulfovibrio alaskensis
1.20.4.4 20000
-
 gel filtration Oleidesulfovibrio alaskensis

Organism

EC Number Organism UniProt Comment Textmining
1.20.4.4 Oleidesulfovibrio alaskensis
-
-
-
1.20.4.4 Oleidesulfovibrio alaskensis G20
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.20.4.4 arsenate + thioredoxin
-
 Oleidesulfovibrio alaskensis arsenite + thioredoxin disulfide + H2O
-
 ?
1.20.4.4 arsenate + thioredoxin
-
 Oleidesulfovibrio alaskensis G20 arsenite + thioredoxin disulfide + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.20.4.4 monomer 1 * 16010, calculated, 1 * 16000, SDS-PAGE, 1 * 16008, ESI-microTOF Oleidesulfovibrio alaskensis

Synonyms

EC Number Synonyms Comment Organism
1.20.4.4 ArsC3
-
 Oleidesulfovibrio alaskensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.20.4.4 2.1
-
 arsenate pH 7.6, 37°C, presence of 150 mM NaCl Oleidesulfovibrio alaskensis
1.20.4.4 2.9
-
 arsenate pH 7.6, 37°C, presence of 50 mM K2SO4 Oleidesulfovibrio alaskensis
1.20.4.4 3.2
-
 arsenate pH 7.6, 37°C, presence of 150 mM KCl Oleidesulfovibrio alaskensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.20.4.4 thioredoxin
-
 Oleidesulfovibrio alaskensis

General Information

EC Number General Information Comment Organism
1.20.4.4 physiological function isoform ArsC3 is functional and able to confer arsenate resistance to Escherichia coli Oleidesulfovibrio alaskensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.20.4.4 256
-
 arsenate pH 7.6, 37°C, presence of 150 mM NaCl Oleidesulfovibrio alaskensis
1.20.4.4 409
-
 arsenate pH 7.6, 37°C, presence of 50 mM K2SO4 Oleidesulfovibrio alaskensis
1.20.4.4 531
-
 arsenate pH 7.6, 37°C, presence of 150 mM KCl Oleidesulfovibrio alaskensis