EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.176 | gene cel7A, heterologous expression of wild-type and mutant enzymes in Aspergillus oryzae | Trichoderma reesei |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.176 | W38A | site-directed mutagenesis, Trp38 in the middle of the active tunnel is replaced with an alanine. This mutation weakens complex formation, and the population of substrate-bound W38A is only about half of the wild-type. Nevertheless, the maximal, steady-state rate is twice as high for the variant enzyme compared to the wild-type enzyme | Trichoderma reesei |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.176 | additional information | - |
additional information | comparative analysis of steady-state kinetics, adsorption, and processivity for wild-type enzyme and the W38A variant, full-length enzymes and catalytic domains with substrate Avicel PH 101, detailed overiew | Trichoderma reesei |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.176 | Avicel PH 101 + H2O | Trichoderma reesei | cellobiose production is monitored by an amperometric enzyme biosensor based on cellobiose dehydrogenase from Phanerochaete chrysosporium adsorbed onto the surface of a benzoquinone-modified carbon paste electrode | ? | - |
? | |
3.2.1.176 | cellulose + H2O | Trichoderma reesei | cellobiohydrolases are exo-active glycosyl hydrolases that processively convert cellulose to soluble sugars, typically cellobiose. Occuring opposite effects on binding and activity by the enzyme can be reconciled if the rate-limiting step is after the catalysis (i.e. in the dissociation process), analysis of the rate-limiting step for cellobiohydrolases, overview | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.176 | Trichoderma reesei | G0RVK1 | an anamorph of the fungus Hypocrea jecorina, gene cel7A | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.176 | Avicel PH 101 + H2O | cellobiose production is monitored by an amperometric enzyme biosensor based on cellobiose dehydrogenase from Phanerochaete chrysosporium adsorbed onto the surface of a benzoquinone-modified carbon paste electrode | Trichoderma reesei | ? | - |
? | |
3.2.1.176 | cellulose + H2O | cellobiohydrolases are exo-active glycosyl hydrolases that processively convert cellulose to soluble sugars, typically cellobiose. Occuring opposite effects on binding and activity by the enzyme can be reconciled if the rate-limiting step is after the catalysis (i.e. in the dissociation process), analysis of the rate-limiting step for cellobiohydrolases, overview | Trichoderma reesei | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.176 | Cel7A | - |
Trichoderma reesei |
3.2.1.176 | cellobiohydrolase | - |
Trichoderma reesei |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.176 | 25 | - |
assay at | Trichoderma reesei |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.176 | 5 | - |
assay at | Trichoderma reesei |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.2.1.176 | additional information | enzyme and catalytic tunnel structure, overview | Trichoderma reesei |