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Literature summary extracted from
- Specificity and catalytic mechanism in family 5 uracil DNA glycosylase (2014), J. Biol. Chem., 289, 18413-18426.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.2.15 | expressed in Escherichia coli BH214 cells | Thermus thermophilus |
| 3.2.2.28 | expressed in Escherichia coli BH214 cells | Thermus thermophilus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.2.28 | D75A | the mutant loses xanthine DNA glycosylase activity while retaining partial uracil and hypoxanthine DNA glycosylase activity | Thermus thermophilus |
| 3.2.2.28 | D75N | the mutant loses xanthine DNA glycosylase activity while retaining much of their uracil and hypoxanthine DNA glycosylase activity | Thermus thermophilus |
| 3.2.2.28 | D75Q | the mutant loses xanthine DNA glycosylase activity while retaining much of their uracil and hypoxanthine DNA glycosylase activity | Thermus thermophilus |
| 3.2.2.28 | H190A | inactive | Thermus thermophilus |
| 3.2.2.28 | N120A | the mutant loses xanthine DNA glycosylase activity while retaining partial uracil and hypoxanthine DNA glycosylase activity | Thermus thermophilus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.2.28 | additional information | not inhibited by Ugi peptide | Thermus thermophilus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.2.15 | hypoxanthine-containing double-stranded DNA + H2O | Thermus thermophilus | strongest activity on the G/I base pair but no activity detected on the C/I base pair | hypoxanthine + double-stranded DNA | - |
? |  |
| 3.2.2.15 | hypoxanthine-containing double-stranded DNA + H2O | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | strongest activity on the G/I base pair but no activity detected on the C/I base pair | hypoxanthine + double-stranded DNA | - |
? | |
| 3.2.2.28 | hypoxanthine-mismatched double-stranded DNA + H2O | Thermus thermophilus | the enzyme also acts as a hypoxanthine DNA glycosylase with the strongest activity on the G/I base pair but no activity detected on the C/I base pair | hypoxanthine + double-stranded DNA with abasic site | - |
? | |
| 3.2.2.28 | hypoxanthine-mismatched double-stranded DNA + H2O | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | the enzyme also acts as a hypoxanthine DNA glycosylase with the strongest activity on the G/I base pair but no activity detected on the C/I base pair | hypoxanthine + double-stranded DNA with abasic site | - |
? | |
| 3.2.2.28 | uracil-mismatched double-stranded DNA + H2O | Thermus thermophilus | the enzyme acts as a double-stranded uracil DNA glycosylase with a relatively low activity on the A/U base pair | uracil + double-stranded DNA with abasic site | - |
? | |
| 3.2.2.28 | uracil-mismatched double-stranded DNA + H2O | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | the enzyme acts as a double-stranded uracil DNA glycosylase with a relatively low activity on the A/U base pair | uracil + double-stranded DNA with abasic site | - |
? | |
| 3.2.2.28 | xanthine-mismatched double-stranded DNA + H2O | Thermus thermophilus | - |
xanthine + double-stranded DNA with abasic site | - |
? | |
| 3.2.2.28 | xanthine-mismatched double-stranded DNA + H2O | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
xanthine + double-stranded DNA with abasic site | - |
? | |
| 3.2.2.28 | xanthine-mismatched single-stranded DNA + H2O | Thermus thermophilus | - |
xanthine + single-stranded DNA with abasic site | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.2.15 | Thermus thermophilus | Q5SJ65 | - |
- |
| 3.2.2.15 | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | Q5SJ65 | - |
- |
| 3.2.2.28 | Thermus thermophilus | Q5SJ65 | - |
- |
| 3.2.2.28 | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | Q5SJ65 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.2.28 | HiTrap chelating column chromatography | Thermus thermophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.2.15 | hypoxanthine-containing double-stranded DNA + H2O | strongest activity on the G/I base pair but no activity detected on the C/I base pair | Thermus thermophilus | hypoxanthine + double-stranded DNA | - |
? | |
| 3.2.2.15 | hypoxanthine-containing double-stranded DNA + H2O | strongest activity on the G/I base pair but no activity detected on the C/I base pair | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | hypoxanthine + double-stranded DNA | - |
? | |
| 3.2.2.28 | hypoxanthine-mismatched double-stranded DNA + H2O | the enzyme also acts as a hypoxanthine DNA glycosylase with the strongest activity on the G/I base pair but no activity detected on the C/I base pair | Thermus thermophilus | hypoxanthine + double-stranded DNA with abasic site | - |
? | |
| 3.2.2.28 | hypoxanthine-mismatched double-stranded DNA + H2O | the enzyme also acts as a hypoxanthine DNA glycosylase with the strongest activity on the G/I base pair but no activity detected on the C/I base pair | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | hypoxanthine + double-stranded DNA with abasic site | - |
? | |
| 3.2.2.28 | additional information | the enzyme showed no activity on oxanine-containing DNA substrates, uracil-mismatched single-stranded DNA and hypoxanthine-mismatched single-stranded DNA | Thermus thermophilus | ? | - |
? | |
| 3.2.2.28 | additional information | the enzyme showed no activity on oxanine-containing DNA substrates, uracil-mismatched single-stranded DNA and hypoxanthine-mismatched single-stranded DNA | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ? | - |
? | |
| 3.2.2.28 | uracil-mismatched double-stranded DNA + H2O | the enzyme acts as a double-stranded uracil DNA glycosylase with a relatively low activity on the A/U base pair | Thermus thermophilus | uracil + double-stranded DNA with abasic site | - |
? | |
| 3.2.2.28 | uracil-mismatched double-stranded DNA + H2O | the enzyme acts as a double-stranded uracil DNA glycosylase with a relatively low activity on the A/U base pair | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | uracil + double-stranded DNA with abasic site | - |
? | |
| 3.2.2.28 | xanthine-mismatched double-stranded DNA + H2O | - |
Thermus thermophilus | xanthine + double-stranded DNA with abasic site | - |
? | |
| 3.2.2.28 | xanthine-mismatched double-stranded DNA + H2O | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | xanthine + double-stranded DNA with abasic site | - |
? | |
| 3.2.2.28 | xanthine-mismatched single-stranded DNA + H2O | - |
Thermus thermophilus | xanthine + single-stranded DNA with abasic site | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.2.15 | family 5 UDGb | the enzyme is an uracil, hypoxanthine, and xanthine DNA glycosylase | Thermus thermophilus |
| 3.2.2.15 | hypoxanthine DNA glycosylase | - |
Thermus thermophilus |
| 3.2.2.28 | family 5 UDGb | - |
Thermus thermophilus |
| 3.2.2.28 | family 5 uracil DNA glycosylase | - |
Thermus thermophilus |
| 3.2.2.28 | UDG | - |
Thermus thermophilus |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.2.2.15 | physiological function | a repair enzyme involved in the removal of base damage | Thermus thermophilus |
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