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Literature summary extracted from

  • Wu, D.; Chen, L.; Sun, Q.; Wu, X.; Jia, S.; Meng, A.
    Uracil-DNA glycosylase is involved in DNA demethylation and required for embryonic development in the zebrafish embryo (2014), J. Biol. Chem., 289, 15463-15473.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.27 expressed in Escherichia coli BL21(DE3) cells Danio rerio

Protein Variants

EC Number Protein Variants Comment Organism
3.2.2.27 D132A inactive Danio rerio

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.2.27 nucleus
-
 Danio rerio 5634
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.27 uracil-mismatched double-stranded DNA + H2O Danio rerio
-
 uracil + double-stranded DNA with abasic site
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.27 Danio rerio
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.27 Ni-NTA column chromatography Danio rerio

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.27 blastomere
-
 Danio rerio
-
3.2.2.27 egg
-
 Danio rerio
-
3.2.2.27 embryo
-
 Danio rerio
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.27 uracil-mismatched double-stranded DNA + H2O
-
 Danio rerio uracil + double-stranded DNA with abasic site
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.27 UDG
-
 Danio rerio
3.2.2.27 Unga isoform Danio rerio
3.2.2.27 uracil-DNA glycosylase a isoform Danio rerio

General Information

EC Number General Information Comment Organism
3.2.2.27 malfunction enzyme knockdown in the zebrafish embryo increases global DNA methylation level, inhibits transcription, and causes embryonic lethality Danio rerio
3.2.2.27 physiological function uracil-DNA glycosylase is involved in postfertilization genomic DNA demethylation and zygotic gene transcription and is required for normal embryonic development in the zebrafish embryo. Enzyme-mediated DNA demethylation facilitates histone modifications of chromatin in zebrafish embryos Danio rerio