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Literature summary extracted from

  • Berezniuk, I.; Lyons, P.J.; Sironi, J.J.; Xiao, H.; Setou, M.; Angeletti, R.H.; Ikegami, K.; Fricker, L.D.
    Cytosolic carboxypeptidase 5 removes alpha- and gamma-linked glutamates from tubulin (2013), J. Biol. Chem., 288, 30445-30453.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.17.24 expression in Sf9 cell Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.24 Mus musculus Q09M02
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.17.24 This is a subfamily of enzymes that cleave C-terminal and/or side chain amino acids from tubulins. The dual-specificity enzymes can cleave both alpha- and gamma-linked L-glutamate from tubulins, removing the posttranslationally added polyglutamyl side chains from the C-terminal regions. In addition, the enzyme removes two glutamate residues from the C-terminus of beta-tubulin and detyrosinated alpha-tubulin (from which the C-terminal L-tyrosine has been removed by EC 3.4.17.17, tubulinyl-Tyr carboxypeptidase). The latter is cleaved to delta2-tubulin and further to delta3-tubulin. enzyme additionally cleaves alpha-linked Glu from the C-terminus of alpha-tubulin and alpha-linked Glu residues from the side chain of alpha- and beta-tubulin Mus musculus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.24 beta2b-tubulin + H2O
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 Mus musculus ? incubation with isoform CCP5 causes decreases of the peaks corresponding to multi- and monoglutamylated beta2b-tubulin and a dramatic increase of the peak corresponding to beta2b-tubulin without Glu on the side chain ?
3.4.17.24 DATAEEEGEMYE(gamma-(E)2)-DDDEESEAQGPK + 2 H2O synthetic beta3-tubulin-based 24-residue C-terminal peptide with two side chain Glu residues Mus musculus DATAEEEGEMYEDDDEESEAQGPK + 2 L-Glu about 30% removal of Glu side chains ?
3.4.17.24 DATAEEEGEMYE(gamma-(E)3)-DDDEESEAQGPK + 3 H2O synthetic beta3-tubulin-based 24-residue C-terminal peptide with three side chain Glu residues Mus musculus DATAEEEGEMYEDDDEESEAQGPK + 3 L-Glu
-
 ?
3.4.17.24 DATAEEEGEMYE(gamma-(E)4)-DDDEESEAQGPK + 4 H2O synthetic beta3-tubulin-based 24-residue C-terminal peptide with four side chain Glu residues Mus musculus DATAEEEGEMYEDDDEESEAQGPK + 4 L-Glu
-
 ?
3.4.17.24 DATAEEEGEMYE(gamma-E)-DDDEESEAQGPK + H2O synthetic beta3-tubulin-based 24-residue C-terminal peptide with one side chain Glu residue Mus musculus DATAEEEGEMYEDDDEESEAQGPK + L-Glu complete removal of Glu side chain ?
3.4.17.24 DELTA2-tubulin + H2O i.e. tubulin lacking two C-terminal amino acids Mus musculus DELTA3-tubulin + L-glutamate i.e. tubulin lacking two C-terminal amino acids ?
3.4.17.24 detyrosinated alpha-tubulin + H2O
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 Mus musculus DELTA2-tubulin + L-glutamate treatment with isoform CCP5 leads to a decrease in the peak intensities of the multi- as well as monoglutamylated alpha-tubulin forms and an increase in the peak intensity of the form lacking all Glu residues on the side chain ?
3.4.17.24 additional information isoform CCP5 removes multiple glutamate residues and the branch point glutamate from the side chains of porcine brain alpha- and beta-tubulin. CCP5 excises C-terminal glutamates from detyrosinated alpha-tubulin. The enzyme also removes multiple glutamate residues from side chains and C-termini of paclitaxel-stabilized microtubules. CCP5 both shortens and removes side chain glutamates from synthetic peptides corresponding to the C-terminal region of beta3-tubulin Mus musculus ?
-
 ?
3.4.17.24 polymerized microtubules + H2O polymerized microtubules prepared from MDA-MB-231 cells grown on a coverslip. Isoform CCP5 eliminates GT335 signals and diminishes poly(E) signals on polymerized tubulin Mus musculus ?
-
 ?
3.4.17.24 tubulin + H2O porcine brain tubulin. Isoform CCP5 can process paclitaxel-treated tubulin, and the activity of CCP5 in the presence of paclitaxel is comparable with the activity in the absence of the drug Mus musculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.17.24 CCP5
-
 Mus musculus
3.4.17.24 deglutamylase
-
 Mus musculus