Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Toulabi, L.; Wu, X.; Cheng, Y.; Mao, Y.
    Identification and structural characterization of a Legionella phosphoinositide phosphatase (2013), J. Biol. Chem., 288, 24518-24527.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.64 expressed in Escherichia coli as a His-tagged fusion protein Legionella pneumophila
3.1.3.64 expressed in Escherichia coli as a His-tagged fusion protein Legionella longbeachae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.64 crystal structure of SidP orthologue from Legionella longbeachae reveals that this unique PI-3-phosphatase is composed of three distinct domains: a large catalytic domain, an appendage domain that is inserted into the N-terminal portion of the catalytic domain, and a C-terminalalpha-helical domain Legionella pneumophila

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.64 C554S mutant does not show any activity Legionella pneumophila
3.1.3.64 D559N mutant does not show any activity Legionella pneumophila
3.1.3.64 R560K mutant does not show any activity Legionella pneumophila

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.64 Legionella longbeachae D3HMN4
-
-
3.1.3.64 Legionella longbeachae NSW150 D3HMN4
-
-
3.1.3.64 Legionella pneumophila
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.64
-
 Legionella pneumophila
3.1.3.64
-
 Legionella longbeachae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.64 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate + H2O
-
 Legionella pneumophila 1-phosphatidyl-1D-myo-inositol 5-phosphate + phosphate
-
 ?
3.1.3.64 1-phosphatidyl-1D-myo-inositol 3-phosphate + H2O
-
 Legionella pneumophila 1-phosphatidyl-1D-myo-inositol + phosphate
-
 ?
3.1.3.64 1-phosphatidyl-1D-myo-inositol 3-phosphate + H2O enzyme shows specific enzymatic activity against PI(3)P but no detectable activity against PI(3,5)P2 Legionella longbeachae 1-phosphatidyl-1D-myo-inositol + phosphate
-
 ?
3.1.3.64 1-phosphatidyl-1D-myo-inositol 3-phosphate + H2O enzyme shows specific enzymatic activity against PI(3)P but no detectable activity against PI(3,5)P2 Legionella longbeachae NSW150 1-phosphatidyl-1D-myo-inositol + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.64 SidP
-
 Legionella pneumophila
3.1.3.64 SidP
-
 Legionella longbeachae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.64 37
-
 assay at Legionella pneumophila
3.1.3.64 37
-
 assay at Legionella longbeachae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.64 8
-
 assay at Legionella pneumophila
3.1.3.64 8
-
 assay at Legionella longbeachae

General Information

EC Number General Information Comment Organism
3.1.3.64 malfunction activity of SidP rescues the growth phenotype of a yeast strain defective in PI(3)P phosphatase activity Legionella pneumophila