BRENDA - Enzyme Database show

Nicotinamide inhibits the lysosomal cathepsin B-like protease and kills African trypanosomes

Unciti-Broceta, J.D.; Maceira, J.; Morales, S.; Garcia-Perez, A.; Munoz-Torres, M.E.; Garcia-Salcedo, J.A.; J. Biol. Chem. 288, 10548-10557 (2013)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.4.22.B6
recombinant expression in Pichia pastoris
Trypanosoma brucei
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.22.B6
nicotinamide
complete inhibition at 25 mM
Trypanosoma brucei
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.4.22.B6
lysosome
-
Trypanosoma brucei
5764
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.22.B6
42000
-
x * 42000, recombinant zymogen, SDS-PAGE
Trypanosoma brucei
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.4.22.B6
Trypanosoma brucei
Q6R7Z5
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.4.22.B6
Z-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
732089
Trypanosoma brucei
Z-Arg-Arg + 7-amino-4-methylcoumarin
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.4.22.B6
?
x * 42000, recombinant zymogen, SDS-PAGE
Trypanosoma brucei
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.4.22.B6
37
-
assay at
Trypanosoma brucei
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.22.B6
6
-
assay at
Trypanosoma brucei
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.22.B6
recombinant expression in Pichia pastoris
Trypanosoma brucei
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.22.B6
nicotinamide
complete inhibition at 25 mM
Trypanosoma brucei
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.4.22.B6
lysosome
-
Trypanosoma brucei
5764
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.22.B6
42000
-
x * 42000, recombinant zymogen, SDS-PAGE
Trypanosoma brucei
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.4.22.B6
Z-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
732089
Trypanosoma brucei
Z-Arg-Arg + 7-amino-4-methylcoumarin
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.4.22.B6
?
x * 42000, recombinant zymogen, SDS-PAGE
Trypanosoma brucei
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.4.22.B6
37
-
assay at
Trypanosoma brucei
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.22.B6
6
-
assay at
Trypanosoma brucei
General Information
EC Number
General Information
Commentary
Organism
3.4.22.B6
malfunction
inhibition of the lysosomal cathepsin B-like protease blocks endocytosis causing cell death. Nicotinamide causes cytokinesis defects and cell cycle arrest at G2 phase
Trypanosoma brucei
3.4.22.B6
physiological function
cathepsin B-like is a lysosomal protease responsible for degradation of host-derived growth factors, such as transferrin, in the bloodstream form of Trypanosoma brucei
Trypanosoma brucei
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.4.22.B6
malfunction
inhibition of the lysosomal cathepsin B-like protease blocks endocytosis causing cell death. Nicotinamide causes cytokinesis defects and cell cycle arrest at G2 phase
Trypanosoma brucei
3.4.22.B6
physiological function
cathepsin B-like is a lysosomal protease responsible for degradation of host-derived growth factors, such as transferrin, in the bloodstream form of Trypanosoma brucei
Trypanosoma brucei