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Literature summary extracted from

  • Jung, T.Y.; Li, D.; Park, J.T.; Yoon, S.M.; Tran, P.L.; Oh, B.H.; Janecek, ?.; Park, S.G.; Woo, E.J.; Park, K.H.
    Association of novel domain in active site of archaic hyperthermophilic maltogenic amylase from Staphylothermus marinus (2012), J. Biol. Chem., 287, 7979-7989.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.133 expressed in Escherichia coli MC1061 cells Staphylothermus marinus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.133 sitting drop vapor diffusion method, using 12% (w/v) polyethylene glycol 4000, 2% (v/v) isopropyl alcohol, 0.1 M ADA, pH 6.5, and 0.1 M Li2SO4 Staphylothermus marinus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.133 0.43
-
 alpha-cyclodextrin pH and temperature not specified in the publication Staphylothermus marinus
3.2.1.133 1.48
-
 beta-cyclodextrin pH and temperature not specified in the publication Staphylothermus marinus
3.2.1.133 2.68
-
 gamma-cyclodextrin pH and temperature not specified in the publication Staphylothermus marinus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.133 Staphylothermus marinus A3DM60
-
-
3.2.1.133 Staphylothermus marinus DSM 3639 A3DM60
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.133 Ni-NTA column chromatography Staphylothermus marinus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.133 alpha-cyclodextrin + H2O
-
 Staphylothermus marinus ?
-
 ?
3.2.1.133 alpha-cyclodextrin + H2O
-
 Staphylothermus marinus DSM 3639 ?
-
 ?
3.2.1.133 amylopectin + H2O
-
 Staphylothermus marinus maltose + D-glucose
-
 ?
3.2.1.133 amylopectin + H2O
-
 Staphylothermus marinus DSM 3639 maltose + D-glucose
-
 ?
3.2.1.133 amylose + H2O
-
 Staphylothermus marinus maltose + D-glucose
-
 ?
3.2.1.133 amylose + H2O
-
 Staphylothermus marinus DSM 3639 maltose + D-glucose
-
 ?
3.2.1.133 beta-cyclodextrin + H2O
-
 Staphylothermus marinus ?
-
 ?
3.2.1.133 beta-cyclodextrin + H2O
-
 Staphylothermus marinus DSM 3639 ?
-
 ?
3.2.1.133 gamma-cyclodextrin + H2O
-
 Staphylothermus marinus ?
-
 ?
3.2.1.133 gamma-cyclodextrin + H2O
-
 Staphylothermus marinus DSM 3639 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.133 maltogenic amylase
-
 Staphylothermus marinus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.133 100
-
-
 Staphylothermus marinus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.133 109
-
 109°C melting temperature with enzymatic activity under acidic conditions (pH 3.5-5.0) Staphylothermus marinus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.133 0.59
-
 amylose pH and temperature not specified in the publication Staphylothermus marinus
3.2.1.133 0.8
-
 amylopectin pH and temperature not specified in the publication Staphylothermus marinus
3.2.1.133 1.45
-
 alpha-cyclodextrin pH and temperature not specified in the publication Staphylothermus marinus
3.2.1.133 5.36
-
 beta-cyclodextrin pH and temperature not specified in the publication Staphylothermus marinus
3.2.1.133 29.1
-
 gamma-cyclodextrin pH and temperature not specified in the publication Staphylothermus marinus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.133 3.37
-
 alpha-cyclodextrin pH and temperature not specified in the publication Staphylothermus marinus
3.2.1.133 3.62
-
 beta-cyclodextrin pH and temperature not specified in the publication Staphylothermus marinus
3.2.1.133 10.86
-
 gamma-cyclodextrin pH and temperature not specified in the publication Staphylothermus marinus