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Literature summary extracted from

  • Ishibashi, Y.; Ikeda, K.; Sakaguchi, K.; Okino, N.; Taguchi, R.; Ito, M.
    Quality control of fungus-specific glucosylceramide in Cryptococcus neoformans by endoglycoceramidase-related protein 1 (EGCrP1) (2012), J. Biol. Chem., 287, 368-381.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.62 EDTA about 120% activity at 1 mM Cryptococcus neoformans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.62 expressed in Escherichia coli BL21(DE3) cells Cryptococcus neoformans

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.62 E254Q inactive Cryptococcus neoformans
3.2.1.62 E483Q inactive Cryptococcus neoformans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.62 Ca2+ about 78% residual activity at 1 mM Cryptococcus neoformans
3.2.1.62 Co2+ about 18% residual activity at 1 mM Cryptococcus neoformans
3.2.1.62 Cu2+ about 10% residual activity at 1 mM Cryptococcus neoformans
3.2.1.62 DMSO the enzyme is inhibited by 30-50% (v/v) DMSO Cryptococcus neoformans
3.2.1.62 Fe2+ about 40% residual activity at 1 mM Cryptococcus neoformans
3.2.1.62 Fe3+ about 50% residual activity at 1 mM Cryptococcus neoformans
3.2.1.62 Hg+ complete inhibition at 1 mM Cryptococcus neoformans
3.2.1.62 Mg2+ about 90% residual activity at 1 mM Cryptococcus neoformans
3.2.1.62 Mn2+ about 50% residual activity at 1 mM Cryptococcus neoformans
3.2.1.62 Na+ about 93% residual activity at 1 mM Cryptococcus neoformans
3.2.1.62 Ni2+ about 5% residual activity at 1 mM Cryptococcus neoformans
3.2.1.62 Pb2+ about 10% residual activity at 1 mM Cryptococcus neoformans
3.2.1.62 Triton X-100 strongly inhibits the activity even at a low concentration Cryptococcus neoformans
3.2.1.62 Zn2+ about 8% residual activity at 1 mM Cryptococcus neoformans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.62 0.0058
-
C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide at pH 7.3 and 30°C Cryptococcus neoformans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.62 membrane
-
Cryptococcus neoformans 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.62 additional information no metal ions are required for the activity of isoform EGCrP1 Cryptococcus neoformans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.62 oligoglycosylglucosyl-(1->1)-ceramide + H2O Cryptococcus neoformans
-
ceramide + oligoglycosylglucose
-
?
3.2.1.62 oligoglycosylglucosyl-(1->1)-ceramide + H2O Cryptococcus neoformans H99
-
ceramide + oligoglycosylglucose
-
?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.62 DMSO the enzyme is stimulated by 10-20% (v/v) DMSO Cryptococcus neoformans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.62 Cryptococcus neoformans H1AE12
-
-
3.2.1.62 Cryptococcus neoformans H99 H1AE12
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.62 GSTrap column chromatography Cryptococcus neoformans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.62 C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide + H2O highest activity Cryptococcus neoformans ?
-
?
3.2.1.62 C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide + H2O highest activity Cryptococcus neoformans H99 ?
-
?
3.2.1.62 glucosylceramide + H2O low activity Cryptococcus neoformans ?
-
?
3.2.1.62 glucosylceramide + H2O low activity Cryptococcus neoformans H99 ?
-
?
3.2.1.62 oligoglycosylglucosyl-(1->1)-ceramide + H2O
-
Cryptococcus neoformans ceramide + oligoglycosylglucose
-
?
3.2.1.62 oligoglycosylglucosyl-(1->1)-ceramide + H2O
-
Cryptococcus neoformans H99 ceramide + oligoglycosylglucose
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.62 EGCrP1
-
Cryptococcus neoformans
3.2.1.62 endoglycoceramidase-related protein 1
-
Cryptococcus neoformans
3.2.1.62 glucocerebrosidase
-
Cryptococcus neoformans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.62 30 35
-
Cryptococcus neoformans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.62 15 40 about 50% activity at 15°C, about 65% activity at 20°C, 100% activity at 30-35°C, about 30% activity at 40°C Cryptococcus neoformans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.62 0.0383
-
C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide at pH 7.3 and 30°C Cryptococcus neoformans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.62 7.5
-
-
Cryptococcus neoformans

General Information

EC Number General Information Comment Organism
3.2.1.62 malfunction gene disruption results in the accumulation of fungus-specific glucosylceramide and immature glucosylceramide that possess sphingoid bases without a methyl substitution concomitant with a dysfunction of polysaccharide capsule formation Cryptococcus neoformans
3.2.1.62 metabolism the enzyme participates in the catabolism of glucosylceramide and especially functions to eliminate immature glucosylceramide in vivo that are generated as by-products due to the broad specificity of glucosylceramide synthase Cryptococcus neoformans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.62 6.6
-
C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide at pH 7.3 and 30°C Cryptococcus neoformans