EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.62 | EDTA | about 120% activity at 1 mM | Cryptococcus neoformans |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.62 | expressed in Escherichia coli BL21(DE3) cells | Cryptococcus neoformans |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.62 | E254Q | inactive | Cryptococcus neoformans |
3.2.1.62 | E483Q | inactive | Cryptococcus neoformans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.62 | Ca2+ | about 78% residual activity at 1 mM | Cryptococcus neoformans | |
3.2.1.62 | Co2+ | about 18% residual activity at 1 mM | Cryptococcus neoformans | |
3.2.1.62 | Cu2+ | about 10% residual activity at 1 mM | Cryptococcus neoformans | |
3.2.1.62 | DMSO | the enzyme is inhibited by 30-50% (v/v) DMSO | Cryptococcus neoformans | |
3.2.1.62 | Fe2+ | about 40% residual activity at 1 mM | Cryptococcus neoformans | |
3.2.1.62 | Fe3+ | about 50% residual activity at 1 mM | Cryptococcus neoformans | |
3.2.1.62 | Hg+ | complete inhibition at 1 mM | Cryptococcus neoformans | |
3.2.1.62 | Mg2+ | about 90% residual activity at 1 mM | Cryptococcus neoformans | |
3.2.1.62 | Mn2+ | about 50% residual activity at 1 mM | Cryptococcus neoformans | |
3.2.1.62 | Na+ | about 93% residual activity at 1 mM | Cryptococcus neoformans | |
3.2.1.62 | Ni2+ | about 5% residual activity at 1 mM | Cryptococcus neoformans | |
3.2.1.62 | Pb2+ | about 10% residual activity at 1 mM | Cryptococcus neoformans | |
3.2.1.62 | Triton X-100 | strongly inhibits the activity even at a low concentration | Cryptococcus neoformans | |
3.2.1.62 | Zn2+ | about 8% residual activity at 1 mM | Cryptococcus neoformans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.62 | 0.0058 | - |
C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide | at pH 7.3 and 30°C | Cryptococcus neoformans |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.62 | membrane | - |
Cryptococcus neoformans | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.62 | additional information | no metal ions are required for the activity of isoform EGCrP1 | Cryptococcus neoformans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.62 | oligoglycosylglucosyl-(1->1)-ceramide + H2O | Cryptococcus neoformans | - |
ceramide + oligoglycosylglucose | - |
? | |
3.2.1.62 | oligoglycosylglucosyl-(1->1)-ceramide + H2O | Cryptococcus neoformans H99 | - |
ceramide + oligoglycosylglucose | - |
? |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.2.1.62 | DMSO | the enzyme is stimulated by 10-20% (v/v) DMSO | Cryptococcus neoformans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.62 | Cryptococcus neoformans | H1AE12 | - |
- |
3.2.1.62 | Cryptococcus neoformans H99 | H1AE12 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.62 | GSTrap column chromatography | Cryptococcus neoformans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.62 | C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide + H2O | highest activity | Cryptococcus neoformans | ? | - |
? | |
3.2.1.62 | C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide + H2O | highest activity | Cryptococcus neoformans H99 | ? | - |
? | |
3.2.1.62 | glucosylceramide + H2O | low activity | Cryptococcus neoformans | ? | - |
? | |
3.2.1.62 | glucosylceramide + H2O | low activity | Cryptococcus neoformans H99 | ? | - |
? | |
3.2.1.62 | oligoglycosylglucosyl-(1->1)-ceramide + H2O | - |
Cryptococcus neoformans | ceramide + oligoglycosylglucose | - |
? | |
3.2.1.62 | oligoglycosylglucosyl-(1->1)-ceramide + H2O | - |
Cryptococcus neoformans H99 | ceramide + oligoglycosylglucose | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.62 | EGCrP1 | - |
Cryptococcus neoformans |
3.2.1.62 | endoglycoceramidase-related protein 1 | - |
Cryptococcus neoformans |
3.2.1.62 | glucocerebrosidase | - |
Cryptococcus neoformans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.62 | 30 | 35 | - |
Cryptococcus neoformans |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.62 | 15 | 40 | about 50% activity at 15°C, about 65% activity at 20°C, 100% activity at 30-35°C, about 30% activity at 40°C | Cryptococcus neoformans |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.62 | 0.0383 | - |
C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide | at pH 7.3 and 30°C | Cryptococcus neoformans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.62 | 7.5 | - |
- |
Cryptococcus neoformans |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.2.1.62 | malfunction | gene disruption results in the accumulation of fungus-specific glucosylceramide and immature glucosylceramide that possess sphingoid bases without a methyl substitution concomitant with a dysfunction of polysaccharide capsule formation | Cryptococcus neoformans |
3.2.1.62 | metabolism | the enzyme participates in the catabolism of glucosylceramide and especially functions to eliminate immature glucosylceramide in vivo that are generated as by-products due to the broad specificity of glucosylceramide synthase | Cryptococcus neoformans |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.62 | 6.6 | - |
C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide | at pH 7.3 and 30°C | Cryptococcus neoformans |