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Literature summary extracted from

  • Ng, K.S.; Leung, H.W.; Wong, P.T.; Low, C.M.
    Cleavage of the NR2B subunit amino terminus of N-methyl-D-aspartate (NMDA) receptor by tissue plasminogen activator: identification of the cleavage site and characterization of ifenprodil and glycine affinities on truncated NMDA receptor (2012), J. Biol. Chem., 287, 25520-25529.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.68 additional information enzyme-mediated degradation of NR2B is plasmin-independent Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.68 additional information no inhibition of cleavage of the N-methyl-D-aspartate receptor NR2B domain by alpha2-antiplasmin, but by a commercial tissue plasminogen activator inhibitor Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.68 extracellular the enzyme can be released into the extracellular space upon depolarizing stimulation Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.68 plasminogen + H2O Homo sapiens activation plasmin + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.68 Homo sapiens P00750
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.68 amygdala
-
 Homo sapiens
-
3.4.21.68 brain
-
 Homo sapiens
-
3.4.21.68 cerebellum
-
 Homo sapiens
-
3.4.21.68 cerebral cortex
-
 Homo sapiens
-
3.4.21.68 glial cell
-
 Homo sapiens
-
3.4.21.68 hippocampus
-
 Homo sapiens
-
3.4.21.68 hypothalamus
-
 Homo sapiens
-
3.4.21.68 microglia
-
 Homo sapiens
-
3.4.21.68 neuron
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.68 methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroanilide + H2O a chromogenic substrate Homo sapiens methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine + p-nitroaniline
-
 ?
3.4.21.68 N-methyl-D-aspartate receptor + H2O usage of total rat brain lysates or MBP-ATD2B fusion proteins, the recombinant human enzyme shows cleavage of the ATD sequence in the NR2B subunit N-terminus of N-methyl-D-aspartate (NMDA) receptor. Enzyme-mediated degradation of NR2B is plasmin-independent. The enzyme cleaves the MBP-ATD2B fusion protein Homo sapiens ? an Arg27-Arg67-truncated NR2B-containing NMDA receptor might be formed ?
3.4.21.68 plasminogen + H2O activation Homo sapiens plasmin + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.68 Tissue plasminogen activator
-
 Homo sapiens
3.4.21.68 tPA
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.68 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.68 7.5
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
3.4.21.68 physiological function the enzyme may directly interact with NR2B subunits of N-methyl-D-aspartate receptors leading to a change in pharmacological properties of NR2B-containing NMDA receptors Homo sapiens