BRENDA - Enzyme Database

Identification of a DELTA4 fatty acid desaturase from Thraustochytrium sp. involved in the biosynthesis of docosahexanoic acid by heterologous expression in Saccharomyces cerevisiae and Brassica juncea

Qiu, X.; Hong, H.; MacKenzie, S.L.; J. Biol. Chem. 276, 31561-31566 (2001)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.19.31
heterologous expression in Saccharomyces cerevisiae and Brassica juncea respectively. Introduction of the full-length enzyme into Brassica juncea under the control of the 35S promoter results in the production of (4Z,7Z,10Z,13Z,16Z,19Z)-docosa-4,7,10,13,16,19-hexaeno in leaves when exogenously supplied with (7Z,10Z,13Z,16Z,19Z)-docosa-7,10,13,16,19-pentaenoic acid
Thraustochytrium sp.
1.14.19.43
expressed in Saccharomyces cerevisiae and Brassica juncea
Thraustochytrium sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.19.31
49800
-
x * 49800, calculated fropm sequence
Thraustochytrium sp.
1.14.19.43
59100
-
x * 59100, calculated from amino acid sequence
Thraustochytrium sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.43
1-acyl-2-palmitoyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Thraustochytrium sp.
-
1-acyl-2-[(3E)-hexadec-3-enoyl]-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.19.43
1-acyl-2-palmitoyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Thraustochytrium sp. ATCC 21685
-
1-acyl-2-[(3E)-hexadec-3-enoyl]-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.19.31
Thraustochytrium sp.
Q8S3C0
-
-
1.14.19.31
Thraustochytrium sp. ATCC 21685
Q8S3C0
-
-
1.14.19.43
Thraustochytrium sp.
Q853C0
-
-
1.14.19.43
Thraustochytrium sp. ATCC 21685
Q853C0
-
-
Reaction
EC Number
Reaction
Commentary
Organism
1.14.19.31
a (7Z,10Z,13Z,16Z)-docosa-7,10,13,16-tetraenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = a (4Z,7Z,10Z,13Z,16Z)-docosa-4,7,10,13,16-pentaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
(1)
Thraustochytrium sp.
1.14.19.31
a (7Z,10Z,13Z,16Z,19Z)-docosa-7,10,13,16,19-pentaenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = a (4Z,7Z,10Z,13Z,16Z,19Z)-docosa-4,7,10,13,16,19-hexaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
(1)
Thraustochytrium sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.31
a (7Z,10Z,13Z,16Z)-docosa-7,10,13,16-tetraenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
732026
Thraustochytrium sp.
a (4Z,7Z,10Z,13Z,16Z)-docosa-4,7,10,13,16-pentaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
-
-
-
?
1.14.19.31
a (7Z,10Z,13Z,16Z)-docosa-7,10,13,16-tetraenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
732026
Thraustochytrium sp. ATCC 21685
a (4Z,7Z,10Z,13Z,16Z)-docosa-4,7,10,13,16-pentaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
-
-
-
?
1.14.19.31
a (7Z,10Z,13Z,16Z,19Z)-docosa-7,10,13,16,19-pentaenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
732026
Thraustochytrium sp.
a (4Z,7Z,10Z,13Z,16Z,19Z)-docosa-4,7,10,13,16,19-hexaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
-
-
-
?
1.14.19.31
a (7Z,10Z,13Z,16Z,19Z)-docosa-7,10,13,16,19-pentaenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
732026
Thraustochytrium sp. ATCC 21685
a (4Z,7Z,10Z,13Z,16Z,19Z)-docosa-4,7,10,13,16,19-hexaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
-
-
-
?
1.14.19.43
1-acyl-2-palmitoyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
732026
Thraustochytrium sp.
1-acyl-2-[(3E)-hexadec-3-enoyl]-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.19.43
1-acyl-2-palmitoyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
732026
Thraustochytrium sp. ATCC 21685
1-acyl-2-[(3E)-hexadec-3-enoyl]-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.19.43
22:4(n-6) + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
732026
Thraustochytrium sp.
docosa-4,7,10,13,16-pentaenoic acid + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.19.43
22:4(n-6) + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
732026
Thraustochytrium sp. ATCC 21685
docosa-4,7,10,13,16-pentaenoic acid + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.19.43
22:5(n-3) + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
732026
Thraustochytrium sp.
docosa-4,7,10,13,16,19-hexaenoic acid + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.19.43
22:5(n-3) + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
732026
Thraustochytrium sp. ATCC 21685
docosa-4,7,10,13,16,19-hexaenoic acid + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.19.31
?
x * 49800, calculated fropm sequence
Thraustochytrium sp.
1.14.19.43
?
x * 59100, calculated from amino acid sequence
Thraustochytrium sp.
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.19.31
heterologous expression in Saccharomyces cerevisiae and Brassica juncea respectively. Introduction of the full-length enzyme into Brassica juncea under the control of the 35S promoter results in the production of (4Z,7Z,10Z,13Z,16Z,19Z)-docosa-4,7,10,13,16,19-hexaeno in leaves when exogenously supplied with (7Z,10Z,13Z,16Z,19Z)-docosa-7,10,13,16,19-pentaenoic acid
Thraustochytrium sp.
1.14.19.43
expressed in Saccharomyces cerevisiae and Brassica juncea
Thraustochytrium sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.19.31
49800
-
x * 49800, calculated fropm sequence
Thraustochytrium sp.
1.14.19.43
59100
-
x * 59100, calculated from amino acid sequence
Thraustochytrium sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.43
1-acyl-2-palmitoyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Thraustochytrium sp.
-
1-acyl-2-[(3E)-hexadec-3-enoyl]-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.19.43
1-acyl-2-palmitoyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Thraustochytrium sp. ATCC 21685
-
1-acyl-2-[(3E)-hexadec-3-enoyl]-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.31
a (7Z,10Z,13Z,16Z)-docosa-7,10,13,16-tetraenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
732026
Thraustochytrium sp.
a (4Z,7Z,10Z,13Z,16Z)-docosa-4,7,10,13,16-pentaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
-
-
-
?
1.14.19.31
a (7Z,10Z,13Z,16Z)-docosa-7,10,13,16-tetraenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
732026
Thraustochytrium sp. ATCC 21685
a (4Z,7Z,10Z,13Z,16Z)-docosa-4,7,10,13,16-pentaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
-
-
-
?
1.14.19.31
a (7Z,10Z,13Z,16Z,19Z)-docosa-7,10,13,16,19-pentaenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
732026
Thraustochytrium sp.
a (4Z,7Z,10Z,13Z,16Z,19Z)-docosa-4,7,10,13,16,19-hexaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
-
-
-
?
1.14.19.31
a (7Z,10Z,13Z,16Z,19Z)-docosa-7,10,13,16,19-pentaenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
732026
Thraustochytrium sp. ATCC 21685
a (4Z,7Z,10Z,13Z,16Z,19Z)-docosa-4,7,10,13,16,19-hexaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
-
-
-
?
1.14.19.43
1-acyl-2-palmitoyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
732026
Thraustochytrium sp.
1-acyl-2-[(3E)-hexadec-3-enoyl]-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.19.43
1-acyl-2-palmitoyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
732026
Thraustochytrium sp. ATCC 21685
1-acyl-2-[(3E)-hexadec-3-enoyl]-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.19.43
22:4(n-6) + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
732026
Thraustochytrium sp.
docosa-4,7,10,13,16-pentaenoic acid + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.19.43
22:4(n-6) + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
732026
Thraustochytrium sp. ATCC 21685
docosa-4,7,10,13,16-pentaenoic acid + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.19.43
22:5(n-3) + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
732026
Thraustochytrium sp.
docosa-4,7,10,13,16,19-hexaenoic acid + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.19.43
22:5(n-3) + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
732026
Thraustochytrium sp. ATCC 21685
docosa-4,7,10,13,16,19-hexaenoic acid + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.19.31
?
x * 49800, calculated fropm sequence
Thraustochytrium sp.
1.14.19.43
?
x * 59100, calculated from amino acid sequence
Thraustochytrium sp.
General Information
EC Number
General Information
Commentary
Organism
1.14.19.31
metabolism
the enzyme is involved in the biosynthesis of docosahexanoic acid
Thraustochytrium sp.
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.19.31
metabolism
the enzyme is involved in the biosynthesis of docosahexanoic acid
Thraustochytrium sp.