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Literature summary extracted from
- Characterization of an N-glycosylated Bacillus subtilis leucine aminopeptidase expressed in Pichia pastoris (2015), J. Basic Microbiol., 55, 236-246.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.11.10 | expressed in Pichia pastoris | Bacillus subtilis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.10 | DL-dithiothreitol | - |
Bacillus subtilis |  |
| 3.4.11.10 | EDTA | - |
Bacillus subtilis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.10 | 0.97 | - |
leucine-4-nitroaniline | pH 8.5, 60°C | Bacillus subtilis | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.10 | Ca2+ | inhibits activity | Bacillus subtilis | |
| 3.4.11.10 | Ca2+ | weakly inhibits activity | Bacillus subtilis | |
| 3.4.11.10 | Co2+ | enhances activity | Bacillus subtilis | |
| 3.4.11.10 | Cu2+ | weakly inhibits activity | Bacillus subtilis | |
| 3.4.11.10 | Ni2+ | inhibits activity | Bacillus subtilis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.10 | 50000 | - |
SDS-PAGE | Bacillus subtilis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.11.10 | Bacillus subtilis | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.11.10 | glycoprotein | enzyme contains three potential glycosylation sites | Bacillus subtilis |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.11.10 | using ion-exchange chromatography, ultrafiltration and gel filtration | Bacillus subtilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.11.10 | leucine-4-nitroaniline + H2O | enzyme also shows activity towards Arg-4-nitroaniline, Lys-4-nitroaniline and Met-4-nitroaniline | Bacillus subtilis | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.11.10 | leucine aminopeptidase | - |
Bacillus subtilis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.10 | 60 | - |
recombinant protein | Bacillus subtilis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.10 | 30 | 60 | recombinant protein | Bacillus subtilis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.10 | 8.5 | - |
recombinant protein | Bacillus subtilis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.10 | 8 | 9 | recombinant protein | Bacillus subtilis |
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Release 2023.1 (January 2023)
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