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Literature summary extracted from

  • Gomez-Manzo, S.; Chavez-Pacheco, J.L.; Contreras-Zentella, M.; Sosa-Torres, M.E.; Arreguin-Espinosa, R.; Perez de la Mora, M.; Membrillo-Hernandez, J.; Escamilla, J.E.
    Molecular and catalytic properties of the aldehyde dehydrogenase of Gluconacetobacter diazotrophicus, a quinoheme protein containing pyrroloquinoline quinone, cytochrome b, and cytochrome c (2010), J. Bacteriol., 192, 5718-5724.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.5.2 CuSO4 1 mM, cosubstrate ferricyanide, strong inhibition; 1 mM, strong inhibition Gluconacetobacter diazotrophicus
1.2.5.2 HgCl2 1 mM, cosubstrate ferricyanide, strong inhibition; 1 mM, strong inhibition Gluconacetobacter diazotrophicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.5.2 3.3
-
 acetic acid cosubstrate ferricyandie, pH 3.5, 30°C Gluconacetobacter diazotrophicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.5.2 membrane membrane-bound complex Gluconacetobacter diazotrophicus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.5.2 50000
-
 1 * 79700 + 1 * 50000, SDS-PAGE under denaturing conditions Gluconacetobacter diazotrophicus
1.2.5.2 79700
-
 1 * 79700 + 1 * 50000, SDS-PAGE under denaturing conditions Gluconacetobacter diazotrophicus
1.2.5.2 79700
-
 1 * 79700 and 1 * 50000,SDS-PAGE Gluconacetobacter diazotrophicus
1.2.5.2 129000
-
 PAGE Gluconacetobacter diazotrophicus
1.2.5.2 129000
-
 nondenaturing PAGE Gluconacetobacter diazotrophicus

Organism

EC Number Organism UniProt Comment Textmining
1.2.5.2 Gluconacetobacter diazotrophicus
-
-
-
1.2.5.2 Gluconacetobacter diazotrophicus ATCC 49037
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.5.2
-
 Gluconacetobacter diazotrophicus

Storage Stability

EC Number Storage Stability Organism
1.2.5.2 -70°C, stable for several months Gluconacetobacter diazotrophicus
1.2.5.2 -70°C, stable for weeks without appreciable loss of activity Gluconacetobacter diazotrophicus
1.2.5.2 4°C, stable for several weeks Gluconacetobacter diazotrophicus
1.2.5.2 4°C, stable for several weeks without appreciable loss of activity Gluconacetobacter diazotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.5.2 acetaldehyde + 2 ferricyanide + H2O
-
 Gluconacetobacter diazotrophicus acetate + 2 ferrocyanide + 2 H+
-
 ?
1.2.5.2 acetaldehyde + 2 ferricyanide + H2O
-
 Gluconacetobacter diazotrophicus ATCC 49037 acetate + 2 ferrocyanide + 2 H+
-
 ?
1.2.5.2 acetaldehyde + ferricyanide
-
 Gluconacetobacter diazotrophicus acetic acid + ferrocyanide + H+
-
 ?
1.2.5.2 acetaldehyde + ferricyanide
-
 Gluconacetobacter diazotrophicus ATCC 49037 acetic acid + ferrocyanide + H+
-
 ?
1.2.5.2 acetaldehyde + oxidized phenazine methosulfate
-
 Gluconacetobacter diazotrophicus acetic acid + reduced phenazine methosulfate
-
 ?
1.2.5.2 acetaldehyde + oxidized phenazine methosulfate
-
 Gluconacetobacter diazotrophicus ATCC 49037 acetic acid + reduced phenazine methosulfate
-
 ?
1.2.5.2 glutaraldehyde + 2 ferricyanide + H2O 26.4% compared to the activity with acetaldehyde Gluconacetobacter diazotrophicus glutarate + 2 ferrocyanide + 2 H+
-
 ?
1.2.5.2 glutaraldehyde + oxidized phenazine methosulfate 26.5% of the activity with acetaldehyde Gluconacetobacter diazotrophicus glutaric acid + reduced phenazine methosulfate
-
 ?
1.2.5.2 additional information formaldehyde and benzaldehyde are not oxidized at all. Aliphatic alcohols and sugar aldehydes, are not substrates. The enzyme is specific for aliphatic aldehydes (C2-C6) and is able to use ferricyanide or phenazine methosulfate plus 2,6-dichlorophenolindophenol as electron acceptors having optimal pH values of 3.5 and 7.0, respectively. Ubiquinone-1 is also able to accept electrons from the dithionite-reduced enzyme Gluconacetobacter diazotrophicus ?
-
 ?
1.2.5.2 additional information no substrates: formaldehyde, benzaldehyde, aliphatic alcohols, sugar aldehydes Gluconacetobacter diazotrophicus ?
-
 ?
1.2.5.2 additional information formaldehyde and benzaldehyde are not oxidized at all. Aliphatic alcohols and sugar aldehydes, are not substrates. The enzyme is specific for aliphatic aldehydes (C2-C6) and is able to use ferricyanide or phenazine methosulfate plus 2,6-dichlorophenolindophenol as electron acceptors having optimal pH values of 3.5 and 7.0, respectively. Ubiquinone-1 is also able to accept electrons from the dithionite-reduced enzyme Gluconacetobacter diazotrophicus ATCC 49037 ?
-
 ?
1.2.5.2 additional information no substrates: formaldehyde, benzaldehyde, aliphatic alcohols, sugar aldehydes Gluconacetobacter diazotrophicus ATCC 49037 ?
-
 ?
1.2.5.2 propanal + oxidized phenazine methosulfate 72.7% of the activity with acetaldehyde Gluconacetobacter diazotrophicus propanoic acid + reduced phenazine methosulfate
-
 ?
1.2.5.2 propionaldehyde + 2 ferricyanide + H2O 72.7% compared to the activity with acetaldehyde Gluconacetobacter diazotrophicus propionate + 2 ferrocyanide + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.5.2 heterodimer 1 * 79700 + 1 * 50000, SDS-PAGE under denaturing conditions Gluconacetobacter diazotrophicus
1.2.5.2 heterodimer 1 * 79700 and 1 * 50000,SDS-PAGE Gluconacetobacter diazotrophicus

Synonyms

EC Number Synonyms Comment Organism
1.2.5.2 aldehyde dehydrogenase (acceptor)
-
 Gluconacetobacter diazotrophicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.5.2 22000
-
 acetic acid cosubstrate ferricyandie, pH 3.5, 30°C Gluconacetobacter diazotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.5.2 3.5
-
 electron accceptor ferricyanide Gluconacetobacter diazotrophicus
1.2.5.2 3.5
-
 using ferricyanide as an electron acceptor, the enzyme shows an optimum pH of 3.5 that shifts to pH 7.0 when phenazine methosulfate plus 2,6-dichlorophenolindophenol are the electron acceptors Gluconacetobacter diazotrophicus
1.2.5.2 7
-
 electron acceptor phenazine methosulfate plus 2,6-dichlorophenolindophenol Gluconacetobacter diazotrophicus
1.2.5.2 7
-
 using ferricyanide as an electron acceptor, the enzyme shows an optimum pH of 3.5 that shifts to pH 7.0 when phenazine methosulfate plus 2,6-dichlorophenolindophenol are the electron acceptors Gluconacetobacter diazotrophicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.5.2 cytochrome b
-
 Gluconacetobacter diazotrophicus
1.2.5.2 cytochrome b the enzyme complex contains one cytochrome b Gluconacetobacter diazotrophicus
1.2.5.2 cytochrome c the smaller subunit bears three cytochromes c Gluconacetobacter diazotrophicus
1.2.5.2 pyrroloquinoline quinone
-
 Gluconacetobacter diazotrophicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.2.5.2 Gluconacetobacter diazotrophicus isoelectric focusing
-
 5.2

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.5.2 6720
-
 acetic acid cosubstrate ferricyandie, pH 3.5, 30°C Gluconacetobacter diazotrophicus