EC Number | Cloned (Comment) | Organism |
---|---|---|
1.6.1.4 | expression in Escherichia coli | Clostridium kluyveri |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.6.1.4 | 0.025 | - |
NADPH | pH 7, 37°C | Clostridium kluyveri | |
1.6.1.4 | 1 | - |
NAD+ | pH 7, 37°C | Clostridium kluyveri |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.3.1.109 | cytoplasm | - |
Clostridium kluyveri | 5737 | - |
1.6.1.4 | cytoplasm | - |
Clostridium kluyveri | 5737 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.109 | (E)-but-2-enoyl-CoA + NADH + oxidized ferredoxin [iron-sulfur] cluster | Clostridium kluyveri | - |
butanoyl-CoA + NAD+ + reduced ferredoxin [iron-sulfur] cluster | - |
? | |
1.3.1.109 | (E)-but-2-enoyl-CoA + NADH + oxidized ferredoxin [iron-sulfur] cluster | Clostridium kluyveri DSM 555 | - |
butanoyl-CoA + NAD+ + reduced ferredoxin [iron-sulfur] cluster | - |
? | |
1.6.1.4 | NADH + H+ + 2 NADP+ + 2 reduced ferredoxin iron-sulfur cluster | Clostridium kluyveri | the iron-sulfur flavoprotein complex, isolated from the bacterium Clostridium kluyveri, couples the exergonic reduction of NADP+ with reduced ferredoxin and the endergonic reduction of NADP+ with NADH | NAD+ + 2 NADPH + 2 reduced ferredoxin iron-sulfur cluster | - |
r | |
1.6.1.4 | NADH + H+ + 2 NADP+ + 2 reduced ferredoxin iron-sulfur cluster | Clostridium kluyveri DSM 555 | the iron-sulfur flavoprotein complex, isolated from the bacterium Clostridium kluyveri, couples the exergonic reduction of NADP+ with reduced ferredoxin and the endergonic reduction of NADP+ with NADH | NAD+ + 2 NADPH + 2 reduced ferredoxin iron-sulfur cluster | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.109 | Clostridium kluyveri | - |
- |
- |
1.3.1.109 | Clostridium kluyveri DSM 555 | - |
- |
- |
1.6.1.4 | Clostridium kluyveri | A5N5D2 and A5N5D3 | A5N5D2: NfnA, A5N5D3: NfnB | - |
1.6.1.4 | Clostridium kluyveri DSM 555 | A5N5D2 and A5N5D3 | A5N5D2: NfnA, A5N5D3: NfnB | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.6.1.4 | - |
Clostridium kluyveri |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.109 | (E)-but-2-enoyl-CoA + NADH + oxidized ferredoxin [iron-sulfur] cluster | - |
Clostridium kluyveri | butanoyl-CoA + NAD+ + reduced ferredoxin [iron-sulfur] cluster | - |
? | |
1.3.1.109 | (E)-but-2-enoyl-CoA + NADH + oxidized ferredoxin [iron-sulfur] cluster | - |
Clostridium kluyveri DSM 555 | butanoyl-CoA + NAD+ + reduced ferredoxin [iron-sulfur] cluster | - |
? | |
1.6.1.4 | NAD+ + 2 NADPH + 2 oxidized ferredoxin iron-sulfur cluster | in the absence of NAD+, ferredoxin is only very slowly reduced (0.5% of the rate seen in the presence of NAD+). In the absence of ferredoxin, NAD+ is only very slowly reduced (less than 1% of the rate observed in the presence of ferredoxin) | Clostridium kluyveri | NADH + H+ + 2 NADP+ + reduced ferredoxin iron-sulfur cluster | - |
r | |
1.6.1.4 | NAD+ + 2 NADPH + 2 oxidized ferredoxin iron-sulfur cluster | in the absence of NAD+, ferredoxin is only very slowly reduced (0.5% of the rate seen in the presence of NAD+). In the absence of ferredoxin, NAD+ is only very slowly reduced (less than 1% of the rate observed in the presence of ferredoxin) | Clostridium kluyveri DSM 555 | NADH + H+ + 2 NADP+ + reduced ferredoxin iron-sulfur cluster | - |
r | |
1.6.1.4 | NADH + H+ + 2 NADP+ + 2 reduced ferredoxin iron-sulfur cluster | the iron-sulfur flavoprotein complex, isolated from the bacterium Clostridium kluyveri, couples the exergonic reduction of NADP+ with reduced ferredoxin and the endergonic reduction of NADP+ with NADH | Clostridium kluyveri | NAD+ + 2 NADPH + 2 reduced ferredoxin iron-sulfur cluster | - |
r | |
1.6.1.4 | NADH + H+ + 2 NADP+ + 2 reduced ferredoxin iron-sulfur cluster | the iron-sulfur flavoprotein complex, isolated from the bacterium Clostridium kluyveri, couples the exergonic reduction of NADP+ with reduced ferredoxin and the endergonic reduction of NADP+ with NADH | Clostridium kluyveri DSM 555 | NAD+ + 2 NADPH + 2 reduced ferredoxin iron-sulfur cluster | - |
r | |
1.6.1.4 | NADH + H+ + 2 NADP+ + 2 reduced ferredoxin iron-sulfur cluster | the iron-sulfur flavoprotein complex, isolated from the bacterium Clostridium kluyveri, couples the exergonic reduction of NADP+ with reduced ferredoxin and the endergonic reduction of NADP+ with NADH. In the absence of NAD+, ferredoxin is only very slowly reduced (0.5% of the rate seen in the presence of NAD+) | Clostridium kluyveri | NAD+ + 2 NADPH + 2 oxidized ferredoxin iron-sulfur cluster | - |
r | |
1.6.1.4 | NADH + H+ + 2 NADP+ + 2 reduced ferredoxin iron-sulfur cluster | the iron-sulfur flavoprotein complex, isolated from the bacterium Clostridium kluyveri, couples the exergonic reduction of NADP+ with reduced ferredoxin and the endergonic reduction of NADP+ with NADH. In the absence of NAD+, ferredoxin is only very slowly reduced (0.5% of the rate seen in the presence of NAD+) | Clostridium kluyveri DSM 555 | NAD+ + 2 NADPH + 2 oxidized ferredoxin iron-sulfur cluster | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.6.1.4 | heterodimer | - |
Clostridium kluyveri |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.109 | butyryl-CoA dehydrogenase-EtfAB complex | - |
Clostridium kluyveri |
1.3.1.109 | butyryl-coenzyme A dehydrogenase-EtfAB complex | - |
Clostridium kluyveri |
1.6.1.4 | NADH-dependent reduced ferredoxin:NADP+ oxidoreductase | - |
Clostridium kluyveri |
1.6.1.4 | NfnAB | - |
Clostridium kluyveri |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.6.1.4 | 37 | - |
assay at | Clostridium kluyveri |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.6.1.4 | 7 | - |
- |
Clostridium kluyveri |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.109 | NADH | - |
Clostridium kluyveri | |
1.6.1.4 | FAD | iron-sulfur flavoprotein complex. Subunit NfnB contains FAD | Clostridium kluyveri | |
1.6.1.4 | iron-sulfur centre | iron-sulfur flavoprotein complex, subunit NfnA has a predicted [2Fe2S] binding site, subunit NfnB has two predicted [4Fe4S] binding sites. UV-visible spectrum shows that the NfnAB complex contains up to 10 Fe molecules per heterodimer, which is consistent with the presence of two [4Fe4S] and one [2Fe2S] clusters | Clostridium kluyveri |