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Literature summary extracted from

  • Hsu, S.; Chung, Y.; Chang, C.; Sung, H.
    Purification and characterization of two chitosanase isoforms from the sheaths of bamboo shoots (2012), J. Agric. Food Chem., 60, 649-657.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.132 diethyl dicarbonate isoform A shows 111% activity at 2.5 mM Bambusa oldhamii
3.2.1.132 p-hydroxymercuribenzoate isoform A shows 112% activity at 0.5 mM Bambusa oldhamii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.132 2,4-Dinitro-1-fluorobenzene isoform A shows 95% residual activity at 0.625 mM, isoform B shows 84% residual activity at 0.625 mM Bambusa oldhamii
3.2.1.132 Ag+ isoform A shows 91% residual activity at 1 mM, isoform B shows 86% residual activity at 1 mM Bambusa oldhamii
3.2.1.132 ethyl acetimidate isoform B shows 56% residual activity at 250 mM Bambusa oldhamii
3.2.1.132 Hg2+ isoform B shows 77% residual activity at 0.25 mM Bambusa oldhamii
3.2.1.132 additional information not inhibited by diethyl dicarbonate, 1,2-cyclohexanedione, and p-hydroxymercuribenzoate Bambusa oldhamii
3.2.1.132 N-Acetylimidazole isoform A shows 96% residual activity at 5 mM, isoform B shows 84% residual activity at 5 mM Bambusa oldhamii
3.2.1.132 N-bromosuccinimide isoform A shows 12% residual activity at 2.5 mM, isoform B is completely inhibited at 2.5 mM Bambusa oldhamii
3.2.1.132 phenylmethylsulfonyl fluoride isoform B shows 86% residual activity at 2.5 mM Bambusa oldhamii
3.2.1.132 Woodward's reagent K N-ethyl-5-phenylisoazoline-3'-sulfonate, isoform A shows 95% residual activity at 50 mM, isoform B shows 1% residual activity at 50 mM Bambusa oldhamii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.132 Ca2+ isoform B shows 117% residual activity at 1 mM Bambusa oldhamii
3.2.1.132 additional information not influenced by Cu2+, Mg2+, K+, and EDTA Bambusa oldhamii
3.2.1.132 Na+ isoform A shows 111% activity at 5 mM Bambusa oldhamii
3.2.1.132 Zn2+ isoform B shows 111% residual activity at 1 mM Bambusa oldhamii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 16400
-
 x * 16400, isoform B, SDS-PAGE Bambusa oldhamii
3.2.1.132 16400
-
 x * 24500 + x * 16400, purified isoform A is composed of a major and a minor chitosanase isoform, SDS-PAGE Bambusa oldhamii
3.2.1.132 24500
-
 x * 24500 + x * 16400, purified isoform A is composed of a major and a minor chitosanase isoform, SDS-PAGE Bambusa oldhamii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.132 chitosan + H2O Bambusa oldhamii chitosan polymers with various degrees of deacetylation (10-94%) are susceptible to hydrolysis by both isoforms. Polymers with 50-70% deacetylation are most susceptible to hydrolysis by isoform A and polymers with 40-80% deacetylation are most susceptible to isoform B D-glucosamine + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Bambusa oldhamii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132 ammonium sulfate precipitation and Sephacryl S-100 gel filtration Bambusa oldhamii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.132 shoot
-
 Bambusa oldhamii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.132 0.0065
-
 crude extract, at pH and °C Bambusa oldhamii
3.2.1.132 0.243
-
 isoform B, after 115.1fold purification, at pH 4.5 and 50°C Bambusa oldhamii
3.2.1.132 0.753
-
 isoform A, after 115.1fold purification, at pH 4.5 and 50°C Bambusa oldhamii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 carboxymethyl chitin + H2O isoform A shows 41.8% activity and isoform B 17.2% activity compared to chitosan Bambusa oldhamii ?
-
 ?
3.2.1.132 chitin + H2O isoform A shows 7.8% activity and isoform B 10.8% activity compared to chitosan Bambusa oldhamii ?
-
 ?
3.2.1.132 chitosan + H2O chitosan polymers with various degrees of deacetylation (10-94%) are susceptible to hydrolysis by both isoforms. Polymers with 50-70% deacetylation are most susceptible to hydrolysis by isoform A and polymers with 40-80% deacetylation are most susceptible to isoform B Bambusa oldhamii D-glucosamine + ?
-
 ?
3.2.1.132 glycol chitin + H2O isoform A shows 29.7% activity and isoform B 14.1% activity compared to chitosan Bambusa oldhamii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.132 ? x * 16400, isoform B, SDS-PAGE Bambusa oldhamii
3.2.1.132 ? x * 24500 + x * 16400, purified isoform A is composed of a major and a minor chitosanase isoform, SDS-PAGE Bambusa oldhamii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 60
-
 isoform B Bambusa oldhamii
3.2.1.132 70
-
 isoform A Bambusa oldhamii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132 30 80 isoform A is stable between 30 and 80°C, whereas isoform B is stable between 30 and 50°C after 60 min incubation Bambusa oldhamii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 3 4
-
 Bambusa oldhamii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.132 3 6 both purified isoforms A and B retain approximately 70-100% relative activity between pH 3.0 and 6.0 but rapidly lose activity above pH 6.0 Bambusa oldhamii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.132 Bambusa oldhamii isoform A, isoelectric focusing
-
 4.3
3.2.1.132 Bambusa oldhamii isoform B, isoelectric focusing
-
 9.2