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Literature summary extracted from

  • Liang, T.W.; Chen, Y.Y.; Pan, P.S.; Wang, S.L.
    Purification of chitinase/chitosanase from Bacillus cereus and discovery of an enzyme inhibitor (2014), Int. J. Biol. Macromol., 63, 8-14.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.132 Cu2+ 8.1% residual activity at 5 mM Bacillus cereus
3.2.1.132 EDTA 73.4% residual activity at 5 mM Bacillus cereus
3.2.1.132 Fe2+ 18.7% residual activity at 5 mM Bacillus cereus
3.2.1.132 Mn2+ 2.3% residual activity at 5 mM Bacillus cereus
3.2.1.132 additional information not inhibited by phenylmethylsulfonyl fluoride, TKUPSP018, TKUPSP073, TKUPSP011, TKUPSP035, TKUPSP080, and TKUPSP062 Bacillus cereus
3.2.1.132 SDS 51.3% residual activity at 2 mM Bacillus cereus
3.2.1.132 TKUPSP017 chitosanase is inhibited completely by 0.05% (w/v) TKUPSP017 Bacillus cereus
3.2.1.132 TKUPSP074 chitosanase is inhibited completely by 0.05% (w/v) TKUPSP074 Bacillus cereus
3.2.1.132 TritonX-100 48.4% residual activity at 1% (v/v) Bacillus cereus
3.2.1.132 Tween 20 57.2% residual activity at 0.5% (v/v) Bacillus cereus
3.2.1.132 Tween 40 51.2% residual activity at 1% (v/v) Bacillus cereus
3.2.1.132 Zn2+ 41.5% residual activity at 5 mM Bacillus cereus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 43000
-
 x * 43000, SDS-PAGE Bacillus cereus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.132 chitosan + H2O Bacillus cereus 100% activity with chitosan of 60% degrees of deacetylation ?
-
 ?
3.2.1.132 chitosan + H2O Bacillus cereus TKU030 100% activity with chitosan of 60% degrees of deacetylation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Bacillus cereus
-
-
-
3.2.1.132 Bacillus cereus TKU030
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132 ammonium sulfate precipitation, DEAE-Sepharose CL-6B column chromatography, and Sephacryl S-100 gel filtration Bacillus cereus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.132 0.002
-
 culture supernatant, at pH 4.0 and 40°C Bacillus cereus
3.2.1.132 0.177
-
 after 88.5fold purification, at pH 4.0 and 40°C Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 alpha-chitin + H2O 11.8% activity compared to chitosan Bacillus cereus ?
-
 ?
3.2.1.132 alpha-chitin + H2O 11.8% activity compared to chitosan Bacillus cereus TKU030 ?
-
 ?
3.2.1.132 beta-chitin + H2O 68.7% activity compared to chitosan Bacillus cereus ?
-
 ?
3.2.1.132 beta-chitin + H2O 68.7% activity compared to chitosan Bacillus cereus TKU030 ?
-
 ?
3.2.1.132 carboxylmethyl cellulose + H2O 61.9% activity compared to chitosan Bacillus cereus ?
-
 ?
3.2.1.132 carboxylmethyl cellulose + H2O 61.9% activity compared to chitosan Bacillus cereus TKU030 ?
-
 ?
3.2.1.132 chitosan + H2O 100% activity with chitosan of 60% degrees of deacetylation Bacillus cereus ?
-
 ?
3.2.1.132 chitosan + H2O 100% activity with chitosan of 60% degrees of deacetylation Bacillus cereus TKU030 ?
-
 ?
3.2.1.132 additional information no activity with colloidal chitin Bacillus cereus ?
-
 ?
3.2.1.132 additional information no activity with colloidal chitin Bacillus cereus TKU030 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.132 ? x * 43000, SDS-PAGE Bacillus cereus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 40
-
-
 Bacillus cereus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132 25 50 the enzyme is stable between 25 and 50°C, maintains its initial activity from 25 to 40°C and has 50% of its activity at 50°C, and is inactivated at 80°C Bacillus cereus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 4
-
-
 Bacillus cereus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.132 3 7 the enzyme is stable between pH 3.0 and 7.0 Bacillus cereus