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Literature summary extracted from

  • Dahech, I.; Ben Ayed, H.; Belghith, K.S.; Belghith, H.; Mejdoub, H.
    Microbial production of levanase for specific hydrolysis of levan (2013), Int. J. Biol. Macromol., 60, 128-133.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.65 2-mercaptoethanol slight activation at 75 mM uncultured bacterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.65 EDTA slight inhibition at 75 mM uncultured bacterium
3.2.1.65 Fe2+ slight inhibition at 75 mM uncultured bacterium
3.2.1.65 Mg2+ slight inhibition at 75 mM uncultured bacterium
3.2.1.65 Zn2+ slight inhibition at 75 mM uncultured bacterium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.65 extracellular
-
 uncultured bacterium
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.65 Mn2+ slight activation at 75 mM uncultured bacterium
3.2.1.65 NaCl slight activation at 75 mM uncultured bacterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.65 levan + H2O uncultured bacterium
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.65 uncultured bacterium
-
 isolated from a Tunisian thermal source
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.65 native extracellular enzyme 397fold from culture supernatant by ammonium sulfate fractionation, dialysis, gel filtration, and anion exchange chromatography uncultured bacterium

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.65 additional information maximum production of levanase activity is achieved after 36 h uncultured bacterium
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.65 226.7
-
 purified native enzyme, pH 6.5, 40°C uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.65 levan + H2O
-
 uncultured bacterium ?
-
 ?
3.2.1.65 levan + H2O
-
 uncultured bacterium levanbiose + D-fructose final products, thinlayer chromatography analysis ?
3.2.1.65 additional information the exo-acting enzyme is capable of hydrolyzing not only beta-2,6-fructosyl linkages, but also beta-2,1-fructosyl linkages uncultured bacterium ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.65 2,6-beta-D fructan fructohydrolase
-
 uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.65 40
-
-
 uncultured bacterium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.65 20 60 activity range uncultured bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.65 40
-
 crude extracellular enzyme, pH 6.4, stable up to uncultured bacterium
3.2.1.65 70
-
 crude extracellular enzyme, pH 6.4, inactivation uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.65 6.2
-
-
 uncultured bacterium