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Literature summary extracted from

  • Khemakhem, B.; Fendri, I.; Dahech, I.; Belghuith, K.; Kammoun, R.; Mejdoub, H.
    Purification and characterization of a maltogenic amylase from Fenugreek (Trigonella foenum graecum) seeds using the Box Benkhen Design (BBD) (2013), Ind. Crops Prod., 43, 334-339.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.133 Ca2+ partial inactivation Trigonella foenum-graecum
3.2.1.133 Mn2+ strong inhibition Trigonella foenum-graecum
3.2.1.133 Zn2+ strong inhibition Trigonella foenum-graecum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.133 Co2+ increases the activity at low concentrations (1 and 2 mM) Trigonella foenum-graecum
3.2.1.133 Fe2+ increases the activity at low concentrations (1 and 2 mM) Trigonella foenum-graecum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.133 68000
-
 x * 68000, SDS-PAGE Trigonella foenum-graecum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.133 starch + H2O Trigonella foenum-graecum
-
 maltose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.133 Trigonella foenum-graecum
-
 fenugreek
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.133 ammonium sulfate precipitation and MonoQ Sepharose column chromatography Trigonella foenum-graecum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.133 seed
-
 Trigonella foenum-graecum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.133 starch + H2O
-
 Trigonella foenum-graecum maltose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.133 ? x * 68000, SDS-PAGE Trigonella foenum-graecum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.133 maltogenic amylase
-
 Trigonella foenum-graecum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.133 60
-
-
 Trigonella foenum-graecum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.133 40 65 more than 50% activity between 40 and 65°C Trigonella foenum-graecum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.133 60
-
 the enzyme is rapidly inactivated at temperature above 60°C and is totally inactivated at 60°C within 60 min in the absence of the substrate. However, in the presence of 1% (w/v) of soluble starch, the half time rises from approximately 10 to 60 min at 60°C Trigonella foenum-graecum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.133 5
-
-
 Trigonella foenum-graecum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.133 4 8 more than 70% activity between pH 4.0 and 7.0, about 40% activity at pH 8.0 Trigonella foenum-graecum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.133 5 10 the enzyme retains 100% of its activity when incubated for 24 h in a pH range between 5.0 and 10.0 Trigonella foenum-graecum