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Literature summary extracted from

  • Martinez, D.; Cutino-Avila, B.; Perez, E.R.; Menendez, C.; Hernandez, L.; Del Monte-Martinez, A.
    A thermostable exo-beta-fructosidase immobilised through rational design (2014), Food Chem., 145, 826-831.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.80 recombinant expression of the C-terminally His-tagged extracellular enzyme in Pichia pastoris strain PpBfrA(4X), secretion to the culture medium, fed-batch fermentation Thermotoga maritima

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.80 additional information immobilisation of the purified recombinant N-glycosylated enzyme designed to incorporate the optimal amount of protein/activity per surface area of aldehyde-activated glyoxyl-resin at pH 8.0-10.0, method overview Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.80 50000
-
x * 50000, deglycosylated recombinant enzyme, SDS-PAGE Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.80 additional information Thermotoga maritima the enzyme shows invertase activity releasing fructose and glucose from sucrose, EC 3.2.1.26 ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.80 Thermotoga maritima
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.80 glycoprotein removal of N-linked oligosaccharides by endoglycoside H treatment Thermotoga maritima

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.80 recombinant extracellular His-tagged enzyme from Pichia pastoris strain PpBfrA(4X) culture supernatant by nickel affinity chromatography Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.80 additional information the enzyme shows invertase activity releasing fructose and glucose from sucrose, EC 3.2.1.26 Thermotoga maritima ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.80 ? x * 50000, deglycosylated recombinant enzyme, SDS-PAGE Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
3.2.1.80 BfrA
-
Thermotoga maritima
3.2.1.80 exo-beta-fructosidase
-
Thermotoga maritima
3.2.1.80 invertase
-
Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.80 60
-
-
Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.80 60 80 the recombinant immonbilized enzyme retains 88%, 77%, and 57% of its initial activity after preincubation for 10 days at temperatures of 60°C, 70°C, and 80°C, respectively. The soluble enzyme is inactivated at a higher rate, overview Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.80 5.5
-
-
Thermotoga maritima