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Literature summary extracted from

  • Esti, M.; Benucci, I.; Liburdi, K.; Garzillo, A.
    Immobilized pineapple stem bromelain activity in a wine-like medium: Effect of inhibitors (2015), Food Bioprod. Process., 93, 84-89.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.4.22.32 food industry the immobilized stem bromelain has productive biotechnological applications in wine-making Ananas comosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.32 ethanol competitive inhibition Ananas comosus
3.4.22.32 additional information grape skin and seed tannins exert uncompetitive inhibition Ananas comosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.32 0.03919
-
 Bz-Phe-Val-Arg-4-nitroanilide chitosan-immobilized enzyme, at pH 3.2 and 25°C Ananas comosus
3.4.22.32 0.0495
-
 Bz-Phe-Val-Arg-4-nitroanilide chitosan-immobilized enzyme, in the presence of 12% (v/v) ethanol, at pH 3.2 and 25°C Ananas comosus
3.4.22.32 0.05731
-
 Bz-Phe-Val-Arg-4-nitroanilide chitosan-immobilized enzyme, in the presence of 18% (v/v) ethanol, at pH 3.2 and 25°C Ananas comosus
3.4.22.32 0.118
-
 Bz-Phe-Val-Arg-4-nitroanilide free enzyme, at pH 3.2 and 25°C Ananas comosus
3.4.22.32 0.25
-
 Bz-Phe-Val-Arg-4-nitroanilide free enzyme, in the presence of 12% (v/v) ethanol, at pH 3.2 and 25°C Ananas comosus
3.4.22.32 0.305
-
 Bz-Phe-Val-Arg-4-nitroanilide free enzyme, in the presence of 18% (v/v) ethanol, at pH 3.2 and 25°C Ananas comosus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.32 Ananas comosus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.32 stem
-
 Ananas comosus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.32 Bz-Phe-Val-Arg-4-nitroanilide + H2O
-
 Ananas comosus Bz-Phe-Val-Arg + 4-nitroaniline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.32 Bromelain
-
 Ananas comosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.32 0.29
-
 Bz-Phe-Val-Arg-4-nitroanilide chitosan-immobilized enzyme, in the presence of 12% (v/v) ethanol, at pH 3.2 and 25°C Ananas comosus
3.4.22.32 0.399
-
 Bz-Phe-Val-Arg-4-nitroanilide chitosan-immobilized enzyme, in the presence of 18% (v/v) ethanol, at pH 3.2 and 25°C Ananas comosus
3.4.22.32 0.461
-
 Bz-Phe-Val-Arg-4-nitroanilide chitosan-immobilized enzyme, at pH 3.2 and 25°C Ananas comosus
3.4.22.32 19.44
-
 Bz-Phe-Val-Arg-4-nitroanilide free enzyme, at pH 3.2 and 25°C Ananas comosus
3.4.22.32 20.03
-
 Bz-Phe-Val-Arg-4-nitroanilide free enzyme, in the presence of 12% (v/v) ethanol, at pH 3.2 and 25°C Ananas comosus
3.4.22.32 21.45
-
 Bz-Phe-Val-Arg-4-nitroanilide free enzyme, in the presence of 18% (v/v) ethanol, at pH 3.2 and 25°C Ananas comosus