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Literature summary extracted from

  • Pillay, D.; Boulange, A.F.; Coustou, V.; Baltz, T.; Coetzer, T.H.
    Recombinant expression and biochemical characterisation of two alanyl aminopeptidases of Trypanosoma congolense (2013), Exp. Parasitol., 135, 675-684.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.2 expressed in Escherichia coli Trypanosoma congolense

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.2 1,10-phenanthroline
-
 Trypanosoma congolense
3.4.11.2 amastatin
-
 Trypanosoma congolense
3.4.11.2 bestatin
-
 Trypanosoma congolense
3.4.11.2 EDTA
-
 Trypanosoma congolense
3.4.11.2 puromycin
-
 Trypanosoma congolense

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.2 0.0329
-
 L-Leu-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense
3.4.11.2 0.04862
-
 L-Leu-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense
3.4.11.2 0.0579
-
 Gly-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense
3.4.11.2 0.08295
-
 Gly-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense
3.4.11.2 0.086
-
 L-Met-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense
3.4.11.2 0.098
-
 L-Ala-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense
3.4.11.2 0.139
-
 L-Ala-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense
3.4.11.2 0.164
-
 L-Met-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.2 cytoplasm
-
 Trypanosoma congolense 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.2 Ca2+ 10 mM, relative activity: 121%, compared to no cation: 100% Trypanosoma congolense
3.4.11.2 Ca2+ 10 mM, relative activity: 137%, compared to no cation: 100% Trypanosoma congolense
3.4.11.2 Co2+ 10 mM, relative activity: 151%, compared to no cation: 100% Trypanosoma congolense
3.4.11.2 Co2+ 10 mM, relative activity: 84%, compared to no cation: 100% Trypanosoma congolense
3.4.11.2 Cu2+ 10 mM, leads to complete inhibition Trypanosoma congolense
3.4.11.2 Cu2+ 10 mM, relative activity: 12%, compared to no cation: 100% Trypanosoma congolense
3.4.11.2 Fe2+ 10 mM, relative activity: 126%, compared to no cation: 100% Trypanosoma congolense
3.4.11.2 Fe2+ 10 mM, relative activity: 139%, compared to no cation: 100% Trypanosoma congolense
3.4.11.2 Mg2+ 10 mM, relative activity: 107%, compared to no cation: 100% Trypanosoma congolense
3.4.11.2 Mg2+ 10 mM, relative activity: 149%, compared to no cation: 100% Trypanosoma congolense
3.4.11.2 Mn2+ 10 mM, relative activity: 140%, compared to no cation: 100% Trypanosoma congolense
3.4.11.2 Mn2+ 10 mM, relative activity: 158%, compared to no cation: 100% Trypanosoma congolense
3.4.11.2 Ni2+ 10 mM, relative activity: 133%, compared to no cation: 100% Trypanosoma congolense
3.4.11.2 Ni2+ 10 mM, relative activity: 141%, compared to no cation: 100% Trypanosoma congolense
3.4.11.2 Zn2+ 10 mM, relative activity: 117%, compared to no cation: 100% Trypanosoma congolense
3.4.11.2 Zn2+ 10 mM, relative activity: 84%, compared to no cation: 100% Trypanosoma congolense

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.2 91000
-
 SDS-PAGE Trypanosoma congolense

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.2 Trypanosoma congolense F9W8I6
-
-
3.4.11.2 Trypanosoma congolense G0UZY5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.2 Gly-7-amido-4-methylcoumarin + H2O
-
 Trypanosoma congolense Gly + 7-amino-4-methylcoumarin
-
 ?
3.4.11.2 L-Ala-7-amido-4-methylcoumarin + H2O
-
 Trypanosoma congolense L-Ala + 7-amino-4-methylcoumarin
-
 ?
3.4.11.2 L-Leu-7-amido-4-methylcoumarin + H2O
-
 Trypanosoma congolense L-Leu + 7-amino-4-methylcoumarin
-
 ?
3.4.11.2 L-Met-7-amido-4-methylcoumarin + H2O
-
 Trypanosoma congolense L-Met + 7-amino-4-methylcoumarin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.2 alanyl aminopeptidase
-
 Trypanosoma congolense
3.4.11.2 Ap1
-
 Trypanosoma congolense
3.4.11.2 Ap2
-
 Trypanosoma congolense

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.2 37
-
 assay at Trypanosoma congolense

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.2 8
-
-
 Trypanosoma congolense

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.11.2 0.00000014
-
 EDTA pH 8, 37°C Trypanosoma congolense
3.4.11.2 0.0000003
-
 EDTA pH 8, 37°C Trypanosoma congolense
3.4.11.2 0.0000024
-
 amastatin pH 8, 37°C Trypanosoma congolense
3.4.11.2 0.0000034
-
 bestatin pH 8, 37°C Trypanosoma congolense
3.4.11.2 0.0000086
-
 amastatin pH 8, 37°C Trypanosoma congolense
3.4.11.2 0.034
-
 1,10-phenanthroline pH 8, 37°C Trypanosoma congolense
3.4.11.2 0.048
-
 1,10-phenanthroline pH 8, 37°C Trypanosoma congolense
3.4.11.2 0.116
-
 puromycin pH 8, 37°C Trypanosoma congolense
3.4.11.2 0.447
-
 puromycin pH 8, 37°C Trypanosoma congolense

General Information

EC Number General Information Comment Organism
3.4.11.2 malfunction down-regulation by RNAi results in a slightly reduced growth rate in procyclic parasites in vitro Trypanosoma congolense

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.11.2 0.83
-
 L-Leu-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense
3.4.11.2 2.21
-
 L-Leu-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense
3.4.11.2 48.96
-
 L-Met-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense
3.4.11.2 69.92
-
 L-Met-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense
3.4.11.2 466
-
 Gly-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense
3.4.11.2 686
-
 Gly-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense
3.4.11.2 1807
-
 L-Ala-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense
3.4.11.2 1853
-
 L-Ala-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense