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Literature summary extracted from

  • Bernard, N.; Johnsen, K.; Ferain, T.; Garmyn, D.; Hols, P.; Holbrook, J.J.; Delcour, J.
    NAD(+)-dependent D-2-hydroxyisocaproate dehydrogenase of Lactobacillus delbrueckii subsp. bulgaricus. Gene cloning and enzyme characterization (1994), Eur. J. Biochem., 224, 439-446.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.345 expression in Escherichia coli Lactobacillus delbrueckii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.345 2-Oxohexanoate substrate inhibition Lactobacillus delbrueckii
1.1.1.345 2-oxopentanoate substrate inhibition Lactobacillus delbrueckii
1.1.1.345 4-methyl-2-oxopentanoate substrate inhibition Lactobacillus delbrueckii
1.1.1.345 phenylpyruvate substrate inhibition Lactobacillus delbrueckii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.345 0.02
-
 2-Oxohexanoate pH 7.5, 25°C Lactobacillus delbrueckii
1.1.1.345 0.022
-
 4-methyl-2-oxopentanoate pH 7.5, 25°C Lactobacillus delbrueckii
1.1.1.345 0.031
-
 phenylpyruvate pH 7.5, 25°C Lactobacillus delbrueckii
1.1.1.345 0.057
-
 2-oxopentanoate pH 7.5, 25°C Lactobacillus delbrueckii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.345 36948
-
 x * 36948, calculated from sequence Lactobacillus delbrueckii

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.345 Lactobacillus delbrueckii Q48534
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.345
-
 Lactobacillus delbrueckii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.345 2-oxohexanoate + NADH + H+ i.e. 2-oxocaproate Lactobacillus delbrueckii 2-hydroxyhexanoate + NAD+
-
 ?
1.1.1.345 2-oxopentanoate + NADH + H+ i.e. 2-oxovalerate Lactobacillus delbrueckii 2-hydroxypentanoate + NAD+
-
 ?
1.1.1.345 4-methyl-2-oxopentanoate + NADH + H+ i.e. 2-oxoisocaproate Lactobacillus delbrueckii (R)-2-hydroxy-4-methylpentanoate + NAD+ i.e. (R)-2-hydroxyisocaproate ?
1.1.1.345 phenylpyruvate + NADH + H+
-
 Lactobacillus delbrueckii phenyllactate + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.345 ? x * 36948, calculated from sequence Lactobacillus delbrueckii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.345 D-2-hydroxyisocaproate dehydrogenase
-
 Lactobacillus delbrueckii
1.1.1.345 HdhD
-
 Lactobacillus delbrueckii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.345 25
-
 assay at Lactobacillus delbrueckii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.345 7.5
-
 assay at Lactobacillus delbrueckii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.345 NADH
-
 Lactobacillus delbrueckii