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Literature summary extracted from

  • Li, H.; Liu, Z.; Wang, C.; Huang, S.; Zhao, M.
    Secretory expression and characterization of a novel thermo-stable, salt-tolerant endo-1,4-beta-mannanase of Bacillus subtilis WD23 by Pichia pastoris (2015), Eur. Food Res. Technol., 240, 671-677.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 cloning in Escherichia coli strain JM109, functional overexpression in Pichia pastoris strain GS115, the enzyme is secreted Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.78 Al3+
-
 Bacillus subtilis
3.2.1.78 Cu2+
-
 Bacillus subtilis
3.2.1.78 EDTA
-
 Bacillus subtilis
3.2.1.78 Fe2+
-
 Bacillus subtilis
3.2.1.78 Fe3+
-
 Bacillus subtilis
3.2.1.78 Hg2+
-
 Bacillus subtilis
3.2.1.78 SDS
-
 Bacillus subtilis
3.2.1.78 Zn2+
-
 Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.78 extracellular the enzyme is secreted Bacillus subtilis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.78 Co2+ activates Bacillus subtilis
3.2.1.78 additional information Na+, Li+, NH4+, Ca2+, Mg2+ and Ba2+ have no effect on the recombinant mannanase activity Bacillus subtilis
3.2.1.78 NaCl activates 150% at 100 mg/ml Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Bacillus subtilis D0UH99 isolated from soil sample of Heilongjiang Liangshui National Nature Reserve
-
3.2.1.78 Bacillus subtilis WD23 D0UH99 isolated from soil sample of Heilongjiang Liangshui National Nature Reserve
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.78 recombinant enzyme 9.7fold from Pichia pastoris strain GS115 culture supernatant to homogeneity by ammonium sulfate fractionation, dialysis, PEG 20000 fractionation, anion exchange chromatography, ultrafiltration, and gel filtration Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.78 4449
-
 purified recombinant enzyme, pH 6.3, 70°C Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.78 endo-1,4-beta-mannanase
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 70
-
 recombinant enzyme Bacillus subtilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.78 40 80 recombinant enzyme, over 80% of maximal activity within this range Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 6.3
-
 recombinant enzyme Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.78 4.8 9.1 recombinant enzyme, more than 80% of the maximum activity at pH 4.8-7.8, the enzyme activity drops rapidly when at below pH 4.8 or above pH 9.1 Bacillus subtilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.78 5.5 7.8 purified recombinant enzyme, over 80% remaning after 1 h Bacillus subtilis
3.2.1.78 6.3 7.1 purified recombinant enzyme, over 80% remaning after 2 h Bacillus subtilis