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Literature summary extracted from

  • Colussi, F.; Garcia, W.; Rosseto, F.; De Mello, B.; De Oliveira Neto, M.; Polikarpov, I.
    Effect of pH and temperature on the global compactness, structure, and activity of cellobiohydrolase Cel7A from Trichoderma harzianum (2012), Eur. Biophys. J., 41, 89-98.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.176 Trichoderma harzianum Q9P8P3
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-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.176 Avicel PH-101 + H2O
-
 Trichoderma harzianum ?
-
 ?
3.2.1.176 crystalline cellulose + H2O
-
 Trichoderma harzianum ?
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 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.176 Cel7A
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 Trichoderma harzianum
3.2.1.176 cellobiohydrolase
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 Trichoderma harzianum
3.2.1.176 ThCel7A
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 Trichoderma harzianum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.176 50
-
 recombinant enzyme Trichoderma harzianum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.176 20 70 recombinant enzyme, activity range, profile overview Trichoderma harzianum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.176 additional information
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 cellobiose-binding influences the enzyme's thermal stability Trichoderma harzianum
3.2.1.176 55 70 thermal unfolding behavior of Th Cel7A is found to be a two-step process at pH 5.0. The first step leads to the formation of molten globule-like states between 55°C and 65°C, followed by a second step that was due to the unfolding of the molten globule-like state at temperatures above 70°C Trichoderma harzianum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.176 5
-
 recombinant enzyme Trichoderma harzianum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.176 3 6 recombinant enzyme, activity range, 76% of maximal activity at pH 3..0, inactive at pH 7.0, profile overview Trichoderma harzianum

General Information

EC Number General Information Comment Organism
3.2.1.176 metabolism the cellobiohydrolase Cel7A is the major component of total extract produced by Trichoderma and is the key enzyme for efficient degradation of crystalline cellulose Trichoderma harzianum
3.2.1.176 additional information tertiary structure analysis at different pH values, small-angle X-ray scattering, overview Trichoderma harzianum