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Literature summary extracted from

  • Gras, S.; Byzia, A.; Gilbert, F.B.; McGowan, S.; Drag, M.; Silvestre, A.; Niepceron, A.; Lecaille, F.; Lalmanach, G.; Brossier, F.
    Aminopeptidase N1 (EtAPN1), an M1 metalloprotease of the apicomplexan parasite Eimeria tenella, participates in parasite development (2014), Eukaryot. Cell, 13, 884-895.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.20 1,10-phenanthroline treatment with 1,10-ortho-phenanthroline in order to deplete metal ions, results in a residual activity of about 8% compared to the initial activity. Activity is partially restored by the addition of divalent metal cations with Zn2+ being the most potent Eimeria tenella

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.20 0.0165
-
 norleucyl-7-amido-4-methylcoumarin pH 7.4, 37°C Eimeria tenella
3.4.11.20 0.0236
-
 (S)-2-amino-4-cyclohexylbutanoyl-7-amido-4-methylcoumarin pH 7.4, 37°C Eimeria tenella
3.4.11.20 0.0262
-
 homophenylalanyl-7-amido-4-methylcoumarin pH 7.4, 37°C Eimeria tenella
3.4.11.20 0.0277
-
 homoargininyl-7-amido-4-methylcoumarin pH 7.4, 37°C Eimeria tenella
3.4.11.20 0.0302
-
 styryl-Ala-7-amido-4-methylcoumarin pH 7.4, 37°C Eimeria tenella
3.4.11.20 0.0314
-
 L-methionyl-7-amido-4-methylcoumarin pH 7.4, 37°C Eimeria tenella
3.4.11.20 0.0354
-
 norvalinyl-7-amido-4-methylcoumarin pH 7.4, 37°C Eimeria tenella
3.4.11.20 0.0598
-
 Ala-7-amido-4-methylcoumarin pH 7.4, 37°C Eimeria tenella

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.20 Zn2+ treatment with 1,10-phenanthroline in order to deplete metal ions, results in a residual activity of about 8% compared to the initial activity. Activity is partially restored by the addition of divalent metal cations with Zn2+ being the most potent Eimeria tenella

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.20 Eimeria tenella V5T9T8
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.11.20 proteolytic modification isoform APN1 is synthesized as a 120-kDa precursor and cleaved into 96-, 68-, and 38-kDa forms during sporulation Eimeria tenella

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.20 (S)-2-amino-4-cyclohexylbutanoyl-7-amido-4-methylcoumarin + H2O
-
 Eimeria tenella (S)-2-amino-4-cyclohexylbutanoic acid + 7-amino-4-methylcoumarin
-
 ?
3.4.11.20 Ala-7-amido-4-methylcoumarin + H2O
-
 Eimeria tenella alanine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.20 homoargininyl-7-amido-4-methylcoumarin + H2O
-
 Eimeria tenella homoarginine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.20 homophenylalanyl-7-amido-4-methylcoumarin + H2O
-
 Eimeria tenella homophenylalanine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.20 L-methionyl-7-amido-4-methylcoumarin + H2O
-
 Eimeria tenella L-methionine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.20 norleucyl-7-amido-4-methylcoumarin + H2O
-
 Eimeria tenella norleucine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.20 norvalinyl-7-amido-4-methylcoumarin + H2O
-
 Eimeria tenella norvaline + 7-amino-4-methylcoumarin
-
 ?
3.4.11.20 styryl-Ala-7-amido-4-methylcoumarin + H2O
-
 Eimeria tenella styryl-alanine + 7-amino-4-methylcoumarin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.20 APN1
-
 Eimeria tenella

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.20 0.456
-
 Ala-7-amido-4-methylcoumarin pH 7.4, 37°C Eimeria tenella
3.4.11.20 0.575
-
 homoargininyl-7-amido-4-methylcoumarin pH 7.4, 37°C Eimeria tenella
3.4.11.20 0.577
-
 styryl-Ala-7-amido-4-methylcoumarin pH 7.4, 37°C Eimeria tenella
3.4.11.20 0.658
-
 norvalinyl-7-amido-4-methylcoumarin pH 7.4, 37°C Eimeria tenella
3.4.11.20 0.704
-
 L-methionyl-7-amido-4-methylcoumarin pH 7.4, 37°C Eimeria tenella
3.4.11.20 0.71
-
 norleucyl-7-amido-4-methylcoumarin pH 7.4, 37°C Eimeria tenella
3.4.11.20 0.737
-
 (S)-2-amino-4-cyclohexylbutanoyl-7-amido-4-methylcoumarin pH 7.4, 37°C Eimeria tenella
3.4.11.20 0.836
-
 homophenylalanyl-7-amido-4-methylcoumarin pH 7.4, 37°C Eimeria tenella

Expression

EC Number Organism Comment Expression
3.4.11.20 Eimeria tenella isoform APN1 is present during the intracellular life cycle stages in both the parasite cytoplasm and the parasite nucleus additional information

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.11.20 7.6
-
 Ala-7-amido-4-methylcoumarin pH 7.4, 37°C Eimeria tenella
3.4.11.20 18.6
-
 norvalinyl-7-amido-4-methylcoumarin pH 7.4, 37°C Eimeria tenella
3.4.11.20 19.1
-
 styryl-Ala-7-amido-4-methylcoumarin pH 7.4, 37°C Eimeria tenella
3.4.11.20 20.8
-
 homoargininyl-7-amido-4-methylcoumarin pH 7.4, 37°C Eimeria tenella
3.4.11.20 22.4
-
 L-methionyl-7-amido-4-methylcoumarin pH 7.4, 37°C Eimeria tenella
3.4.11.20 31.9
-
 homophenylalanyl-7-amido-4-methylcoumarin pH 7.4, 37°C Eimeria tenella
3.4.11.20 43
-
 norleucyl-7-amido-4-methylcoumarin pH 7.4, 37°C Eimeria tenella